益气活血软坚解毒方对荷瘤小鼠抑瘤作用及对生存期影响研究

Effect of Yiqi Huoxue Ruanjian and Jiedu prescriptions on inhibition of tumor growth and survival period in tumor-bearing mice

关键词:  益气活血软坚解毒方  肝癌  抑瘤率  生存期  实验研究

Key words:  YHRJ decoction  hepatocellular  carcinoma  survival time  experimental research

摘要:

[目的]研究益气活血软坚解毒(YHRJ)方对荷瘤小鼠生存期的影响。[方法]抑瘤作用观察:荷S₁₈₀腹水瘤昆明鼠、荷H₂₂昆明鼠与615小鼠,造模后随机分成对照组、CTX(环磷酰胺)组、YHRJ等效剂量组、YHRJ高剂量组、YHRJ等效剂量组+CTX或顺铂(DDP)组、YHRJ高剂量组+CTX(或DDP)组,第2天开始用药,于第13天拉颈处死动物,取出瘤组织,称质量。生存期观察:荷H₂₂昆明鼠,截尾期为对照组出现第1只动物死亡为止,记录小鼠存活时间。[结果]1)对荷S₁₈₀昆明鼠:各用药组肿瘤明显缩小,YHRJ高剂量抑制率为64.22%,与生理盐水及YHRJ等效剂量组相比,差异显著;YHRJ高剂量+CTX组抑制率为80.10%,高于YHRJ等效剂量组及CTX组。2)对荷H₂₂昆明鼠:除YHRJ等效剂量组外,其余各组抑瘤率均超过30%,抑瘤作用明显(P<0.05),YHRJ高剂量组抑瘤率为51.36%。优于其他各组。3)对荷H₂₂615小鼠肿瘤:YHRJ等效剂量抑瘤率为47.15%,YHRJ高剂量+DDP组抑瘤率为80.60%,说明中药对DDP有增效作用。4)对荷H₂₂昆明鼠生存率影响:YHRJ高剂量组及各加DDP组荷瘤小鼠生存期及中位生存天数明显延长,生存率明显提高有统计学意义。[结论]益气活血软坚解毒方对小鼠S₁₈₀腹水瘤、H₂₂肝细胞癌肿瘤生长有抑制作用,并明显延长荷H₂₂昆明鼠生存期。

Abstract:

[Objective] To study the inhibitory effect on growth of tumor and observe the influence on survival period by using the decoction of Yiqi Huoxue Ruanjian Jiedu (YHRJ decoction) in treating the transplanted carcinoma (S₁₈₀ and H₂₂) in mice. [Methods] The mice transplanted with carcinoma (S₁₈₀ or H₂₂) were randomly divided into six groups,including NS control group,CTX or DDP group,YHRJ equivalent-dose group,YHRJ high-dose group,YHRJ equivalent-dose plus CTX (or DDP)group,YHRJ high-dose plus CTX (or DDP)group. All the mice in each group were administered from second day,and killed on the thirteenth day. At the same time the carcinoma was cut down and weighed. Observation of the survival time on Kunming Mice which was transplanted with H₂₂ carcinoma was ended till any one mice died in control group. [Results] In the kunming model mice (S₁₈₀) the weight of carcinoma in all medication groups was lower com-pared with NS control group. The inhibition rate of YHRJ high-dose group was 64.22%,and there was a significant difference when com-paring with NS control group and YHRJ equivalent-dose group. The inhibition rate of YHRJ high-dose plus CTX group was 80.10%,better than YHRJ equivalent-dose group and CTX (or DDP) group. In kunming model mice (H₂₂) the inhibition rate in all medications groups was higher than 30% except for YHRJ equivalent-dose group. The inhibition rate of YHRJ high-dose group was 51.36%,which was higher than other groups. In the model mice (H₂₂615) the inhibition rate of YHRJ equivalent-dose group was 47.15% and that of YHRJ high-dose plus DDP group was 80.60%. It indicated that Chinese herb could reinforce the curative effect. Observation of the influence on survival period showed that the survival time and the mean survival time in YHRJ high-dose group and the groups added by DDP were prolonged. The survival rate was enhanced. [Conclusion] It was proved that the YHRJ decoction could inhibit the growth of transplanted carcinoma (S₁₈₀ or H₂₂) in mice,and could prolong the survival time of kunming model mice.