乌萸汤含药血清对大鼠卵巢颗粒细胞INHB、FSHR mRNA及分泌功能的影响

Effects of Wu Yu decoction drug-containing serum on expression of INHB,FSHR mRNA in ovarian granulose cell and secretion function

关键词:  乌萸汤  卵巢颗粒细胞  抑制素  促卵泡素受体  雌二醇

Key words:  Wu Yu decoction  ovarian granulose cell  INHB  FSHR mRNA  E₂

摘要:

[目的]运用血清药理学方法观察乌萸汤含药血清对大鼠卵巢颗粒细胞抑制素(INHB)、促卵泡激素受体(FSHR)mRNA的表达及其分泌功能的影响。[方法]在大鼠卵巢颗粒细胞培养体系中加入乌萸汤、尿促卵泡激素(FSH)及正常大鼠含药血清,培养48h后,分别用酶联免疫吸附法(ELISA),逆转录聚合酶链式反应(RT-PCR)及放免法测定颗粒细胞INHB、FSHR、mRNA及培养液中雌二醇(E₂)含量。[结果]各用药血清组E₂含量较对照组均明显升高;颗粒细胞INHB、FSHRmRNA表达量明显增多,其中乌萸汤中剂量血清组效果优于西药组。[结论]乌萸汤含药血清能明显促进卵巢颗粒细胞INHB、FSHRmRNA的表达以及E₂分泌,说明乌萸汤可以通过调控卵巢局部因子、增强FSHRmRNA的表达从而提高卵巢对促性腺激素的反应性,改善下丘脑-垂体-卵巢轴,促进E₂的分泌,以促进卵泡发育成熟及排出。

Abstract:

[Objective] To research the effects of Wu Yu decoction drug-containing serum on the expression of INHB,FSHR mRNA in ovarian granulose cell (abbr.GC) and the secretion function in rats. [Methods] Wu Yu decoction,FSH drug-containing serum and the normal rat serum were added into rat ovarian GC culture medium system. They were cultured for 48 hours. The expression of INHB was measured by ELISA,FSHR mRNA expression levels by RT-PCR,E2 levels of each group by radioimmunoassay. [Results] The E2 level was significantly higher in treatment groups than that in control groups,and the expression of INHB,FSHR mRNA significantly increased,among them medium dosage group of Wu Yu decoction was superior than that in Western medicine groups. [Conclusion] Wu Yu decoction drug-containing serum promotes the expression of INHB,FSHR mRNA and secretion of E2 of ovarian GC significantly. It is proved that Wu Yu decoction can enhance the ovarian reaction function to gonadotropins and improve the HPOA through adjusting internal factors of ovary and enhancing the expression of FSHR mRNA,thereby to promote the development and maturation of follicular and ovulation.