心复康联合骨髓间充质干细胞移植对濒死心肌的保护作用

Protective action on impending death cardiac muscles by the therapy of traditional Chinese medicine XinFuKang combined with transplanted bone mesenchymal stem cells

关键词:  心复康  骨髓间充质干细胞  心肌梗死  血管新生

Key words:  XinFuKang  BMSCs  Myocardial infarction  Vessel regeneration

摘要:

[目的]探讨中药心复康联合骨髓间充质干细胞(BMSCs)移植在大鼠急性心肌梗死后对濒死心肌的保护作用及其机制。[方法]经密度梯度离心联合贴壁筛选法培养并纯化BMSCs,采用冠状动脉结扎法建立大鼠急性心肌梗死模型,移植前以5-溴脱氧尿核苷(Brdu)对移植细胞进行标记,术后采用中药心复康灌胃联合干细胞移植治疗。治疗后2周处死动物,免疫组织化学方法检测心肌损伤区Brdu阳性移植细胞数、CD34⁺新生血管数和细胞增殖核抗原(PCNA)阳性心肌细胞数量。[结果]心复康联合干细胞移植治疗组移植细胞存活率增加(与细胞移植组比较P<0.05),梗死边缘区新生血管数量及PCNA阳性心肌细胞数量均有所增加(与细胞移植组比较P<0.05)。[结论]中药心复康联合干细胞移植对大鼠急性心肌梗死后濒死心肌细胞有明显的保护作用,其机制可能与心复康联合干细胞移植能更有效的促进损伤区血管新生有关。

Abstract:

[Objective]To discuss the protective action on impending death cardiac muscles by the therapy of traditional Chinese medicine XinFuKang (XFK) combined with transplanted Bone mesenchymal stem cells (BMSCs) after rats acute myocardial infarction. [Methods] BMSCs were cultured and purified by gradient centrifugation -adhesive method. The rats with acute myocardial infarction were builted by coronary artery ligation. Before transplation,BMSCs were signed by Brdu,and after the OPS. of coronary artery ligation,the therapy methode was XFK combining BMSCs transplantation. After 2 weeks,we measured cell amount of Brdu,neonatal vessels of CD34 and the cardiac muscle of PCNA around the infarct area by method of IHC. [Results] The method of XFK combining BMSCs transplantation can increase livability of transplanted BMSCs,enhance the amount of neonatal vessel and PCNA cardiac muscle around infarct area (compared with group of BMSCs tranplation,P<0.05). [Conclusion] The therapy of XFK combined with BMSCs transplantation have better protection on the impending death cardiac muscles around the infarct area,the mechanism maybe the therapy can promote the vessels regeneration.