| 摘要: |
| [目的]建立有效的控制复方草珊瑚含片质量的方法。[方法]通过高效液相色谱法,SunFireODS色谱柱(4.6mm×150mm,5μm);乙腈-0.1%磷酸溶液梯度洗脱;流速为每分钟0.8mL;柱温为38℃;检测波长294nm。[结果]原儿茶酸、迷迭香酸、3-羟基-4-甲氧基苯甲酸线性范围分别为2.508～50.16ng、41.04～820.8ng、18.40～368.0ng。[结论]该法快速、简便、精密度、重复性、稳定性好。 |
| 关键词: 肿节风 原儿茶酸 3-羟基-4-甲氧基苯甲酸 迷迭香酸 |
| DOI：10.11656/j.issn.1672-1519.2010.02.35 |
| 分类号: |
| 基金项目: |
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| Simultaneous determination of three phenolic acids in Fufang Caoshanhu buccal by HPLC method |
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LI Yi-guang1,2, Lü Yi-bin2, LUO Yue-hua3
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1.Beijing University of Chinese Medicine, Beijing 100029, China;2.Jiangzhong Pharmaceutical Co.LTD., Nanchang 330096, China;3.JianXi Provicial Institate for Drog and Food Contral, Nanchang 330029, China
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| Abstract: |
| [Objective] To establish the method for simultaneous determination of three phenolic acids in Fufang Caoshanhu buccal tablets by high performance liquid chromatograph. [Methods] The HPLC analysis was performed on SunFire ODS column by using gradient elution.The mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution;the detection wavelength was 294 nm;the column temperature was maintained at 38℃;the flow rate was 0.8 ml/min. [Results] Procatechuic acid,Rosmarinic acid,3-Hydroxy-4-methoxy benzoic acid showed good linearity in the correspondent concentration 2.508～50.16 ng,41.04～820.8 ng,18.40～368.0 ng respectively. [Conclusion] The method was accurate,repeatable and simple for analysis of Fufang Caoshanhu buccal tablets. |
| Key words: Sarcandra glabra (Thunb.) Nakai Procatechuic acid 3-Hydroxy-4-methoxy benzoic acid Rosmarinic acid |
