| 本文已被：浏览 1518次 下载 2179次 |
码上扫一扫！ |
|
|
| 红花多糖对人PBMC增殖活性及分泌细胞因子的影响 |
|
石学魁1, 阮殿清2, 陶冀3, 周明瑶3, 王亚贤3
|
|
1.牡丹江医学院病原生物学教研室, 牡丹江, 157011;2.木兰县疾病控制中心, 哈尔滨, 151900;3.黑龙江中医药大学微生物与免疫学教研室, 哈尔滨, 150040
|
|
| 摘要: |
| [目的]探讨红花多糖(SPS)对人外周血单个核细胞(PBMC)的增殖作用及PBMC诱生细胞因子的影响。[方法]采集健康成人外周血,常规分离PBMC,体外与不同浓度的SPS共同培养,检测PBMC的增殖活性及白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)的水平。[结果]SPS对PBMC增殖有促进作用,以1.25g/L和0.625g/L剂量组增殖活性增加明显(P<0.05);红花多糖(SPS)各组IL-2和IFN-γ水平明显高于对照组(P<0.05),而各组TNF-α的水平无显著性差异。[结论]SPS可促进PBMC的增殖,提高IL-2和IFN-γ水平,增强免疫功能。 |
| 关键词: 红花多糖 外周血单个核细胞 增殖 白细胞介素-2 肿瘤坏死因子-α 干扰素-γ |
| DOI：10.11656/j.issn.1672-1519.2010.04.34 |
| 分类号: |
| 基金项目:黑龙江省科技厅基金资助项目(D00-17) |
|
| The influence of SPS on the proliferation of PBMC in vitro and cytokines secretion |
|
SHI Xue-kui1, RUAN Dian-qing2, TAO Ji3
|
|
1.Teaching and Research Section of Pathogenic Biology, Mudanjiang Medical College, Mudanjiang 157011, China;2.Mulan center for disease control and prevention, Harbin 151900, China;3.Department of Microbiology and Immunology, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
|
| Abstract: |
| [Objection] To discuss the influence of SPS on the proliferation of PBMC in vitro and Cytokines secretion. [Methods] PBMC from healthy donors were incubated with different doses of SPS in vitro.The proliferative activity of PBMC and serum IL-2,TNF-α,IFN-γ were measured. [Results] SPS(1.25 g/L and 0.625 g/L dose group) could obviously stimulate the proliferation of PBMC(P<0.05);The level of IL-2,IFN-γ of SPS were dramaticlly higher than those in conrtrol group.(P<0.05).There was no significant difference in TNF-α-concentration in each group. [Conclusion] SPS can promote the proliferation of PBMC and can enhance IL-2 and IFN-γ levels and im-mune function. |
| Key words: Safflower polysaccharide(SPS) Peripheral blood mononuclear cells(PBMC) proliferation IL-2 TNF-α IFN-γ |
