银杏叶提取物EGB761对阿尔茨海默病家兔模型ACAT1和LCAT mRNA表达的影响

Effect of ginkgo biloba extract EGB761 on the mRNA expression of ACAT1 and LCAT in rabbit with Alzheimer’s disease

关键词:  银杏叶提取物EGB761  阿尔茨海默病  卵磷脂-胆固醇酰基转移酶  胆固醇酰基转移酶1

Key words:  ginkgo biloba extract EGB76  Alzheimer’s disease  Lecithin-cholesterol acyl transferase  cholesterol acyltransferase 1

摘要:

[目的]观察银杏叶提取物EGB761对阿尔茨海默(AD)病家兔模型胆固醇酰基转移酶(ACAT1)和卵磷脂-胆固醇酰基转移酶(LCAT)mRNA表达的影响。[方法]以2%高胆固醇饲料喂养雄性新西兰大白兔,建立AD家兔模型。模型建立后,用50 mg/(kg·d)EGB761灌胃3周作为EGB761组,以5 mg/(kg·d)辛伐他汀作为阳性药对照组。干预结束后,通过实时定量逆转录-聚合酶链反应(RT-PCR)法检测海马卵磷脂-胆固醇酰基转移酶mRNA和胆固醇酰基转移酶1 mRNA的表达。[结果]银杏叶提取物EGB761可以下调ACAT1 mRNA的表达,不影响LCAT mRNA的表达。[结论]银杏叶提取物EGB761可下调AD家兔模型ACAT1 mRNA表达,对LCAT mRNA表达无调节作用。

Abstract:

[Objective] To observe whether Ginkgo biloba extract EGB761 can influence the expression of ACAT1 and LCAT mRNA in rabbit with Alzheimer’s disease.[Methods] The male adult New Zealand rabbits were fed by 2% high cholesterol feed in order to establish the model of Alzheimer’s disease.EGB761 was used to treat the rabbits at a dose of 50 mg/(kg·d) for 3 weeks as EGB761 group.Simvastatin was fed at a dose of 5 mg/(kg·d) as a positive control drug.After intervention,the content of lecithin-cholesterol acyl transferase mRNA and cholesterol acyltransferase mRNA in hippocampus was tested by means of real-time quantitative RT-PCR.[Results] The content of ACAT1 mRNA in hippocampus was reduced by Ginkgo biloba extract EGB761,P<0.01 compared with model group.But the content of ACAT1 mRNA was not significant different compared with model group(P>0.05).[Conclusion] Ginkgo biloba extract EGB761 can down-regulate the expression of ACAT1 mRNA in rabbit with Alzheimer’s disease,however,it has no effect on the expression of LCAT mRNA.