三七花治疗高血压病的实验研究

Experimental study on the effect of Sanqi flower in treating hypertension

关键词:  高血压病  三七花  自发性高血压大鼠  一氧化氮  血浆内皮素-1  血管紧张素Ⅱ  降钙素基因相关肽  血栓素B₂  6-酮-前列腺素F_1α

Key words:  hypertension  Sanchi flower  spontaneous hypertension rats  NO  ET-1  AngⅡ  CGRP  TXB₂  6-keto-PGF_1α

摘要:

[目的]本研究观察了传统中药三七花对自发性高血压大鼠(SHR)的血压干预,并初步探讨了其作用机制。[方法]以自发性高血压大鼠(SHR)为实验动物模型。以三七花温浸剂灌服作为干预措施,以松龄血脉康、硝苯地平作为对照。以血压、血浆内皮素-1(ET-1)、一氧化氮(NO)、血管紧张素Ⅱ(AngⅡ)、降钙素基因相关肽(CGRP)、血栓素B₂(TXB₂)、6-酮-前列腺素F_1α(α6-keto-PGF_1α)含量作为观察研究指标。经每日给药,连续8周,期间以无创尾容积法测血压4次,全部给药结束后腹主动脉取血测相关指标。[结果]三七花温浸剂在一定剂量下对SHR具有一定的降压作用。并能降低血浆中ET-1和NO含量。[结论]三七花温浸剂对SHR具有降压作用,其作用机制可能通过改善血管内皮细胞功能,从而使血管收缩因子与血管舒张因子之间的协调趋于平衡有一定关系。

Abstract:

[Objective] This study observed the intervention effect of Sanqi(panax notoginseng) flower,a traditional Chinese medicine,on hypertension in the spontaneous hypertension rats(SHRs) and investigated its mechanism preliminarily.[Methods] We used SHRs as experimental animal model,taking the Sanqi flower infusions as the intervention method and Songling Xuemai Kang capsule and Nifedipine as control and taking blood pressure,ET-1,NO,AngⅡ,CGRP,TXB₂,6-keto-PGF_1α as the observation indexes.The medicines were given daily and for eight weeks.Blood pressure was measured in this duration with the noninvasive tail-volume method four times in all.Finally the other relative blood indexes were measured through sampling from abdominal aorta blood.[Results] 1) The Sanqi flower infusion had corresponding hypotensive effectiveness on certain dose.2) Through the measurement of some plasma active matters it was showed that the hypotensive effectiveness probably had some relation with improvement of vascular endothelial cell function and decreasing plasma ET-1,resuming the balance of vascular constriction and diastolic factors.[Conclusion] The Sanqi flower infusion has corresponding hypotensive effectiveness on hypertension of SHRs.Its hypotensive mechanism probably has some relation with improvement of vascular endothelial cell function and decreasing plasma ET-1,resuming the balance of vascular constriction and diastolic factors.