调气消积汤对Lewis肺癌小鼠免疫功能影响的实验研究

Effects of Tiaoqi xiaoji decoction on immunologic function in mice with Lewis lung cancer

关键词:  调气消积汤  Lewis肺癌小鼠  免疫功能

Key words:  Tiaoqixiaoji decoction  Lewis lung cancer  immunologic function

摘要:

[目的]探讨调气消积汤对Lewis肺癌小鼠免疫功能的影响。[方法]C57BL/6小鼠常规接种Lewis肺癌,随机分为:荷瘤对照组(MG)、调气消积汤组(TG)、顺铂组(DG)、综合组(ZG);健康的小鼠组成空白对照组(CG),每组10只。观察各组对脾脏指数的影响,计数小鼠外周血T淋巴细胞转化率及脾T淋巴细胞的增殖情况,检测肿瘤坏死因子-α(TNF-α)的表达。[结果]调气消积汤可使荷瘤小鼠的脾脏指数降低并趋向正常,与空白组比较无差异(P>0.05)。从小鼠T淋巴细胞转化率及增殖活性来看,调气消积汤组比荷瘤对照组明显升高,差异显著(P<0.01)。调气消积汤组移植瘤TNF-α蛋白的表达,与荷瘤对照组比较无明显差异(P>0.05)。[结论]调气消积汤对Lewis肺癌小鼠的免疫器官(脾)有一定的保护作用;可以通过提高荷瘤小鼠外周血T淋巴细胞转化率、脾T淋巴细胞增殖活性,来增强荷瘤小鼠的免疫功能;对肿瘤组织中TNF-α蛋白的表达无明显促进和抑制作用。

Abstract:

[Objective] To explore the therapeutic effect of Tiaoqixiaoji(regulating Qi and removing food stagnancy) decoction(TQXJ) on the immunologic function in mice with Lewis lung cancer.[Methods] The C57BL/6 mice were inoculated with Lewis lung cancer cells,then randomly divided into: model group(MG),TQXJ group(TG),DDP(Cisplatin) group(DG) and combined TQXJ-DDP group(ZG),with 10 mice in each group;Aanother ten healthy mice were served as control(CG).Different drugs were given to each groups.The index of spleen,the T lymphocyte transformation rate of the peripheral blood,the T lymphocyte proliferation of spleen and expression of tumor necrosis factor-α(TNF-α) of each group were observed.[Results] The decoction of Tiaoqixiaoji could decrease the spleen index of TG and make it proximal to normal value.There was no statistical difference when compared with CG(P>0.05).Observing from the point of view of the T lymphocyte transformation rate and the T lymphocyte proliferation the effect of TG was higher than the MG(P<0.01).The expression of TNF-α was not statistically different between the TG and MG(P>0.05).[Conclusion] TQXJ can protect the immune organ of mice with Lewis lung cancer.It can also improve the immunologic function of the tumor-bearing mice by increasing the T lymphocyte transformation rate of the peripheral blood and the T lymphocyte proliferation of spleen.Hpwever it can not affect the xpression of TNF-α in tumor tissue.