用均匀设计优选栀子柏皮汤半仿生法提取的工艺条件

Optimizing the technological parameters for extracting Zhizi Baipi decoction with uniform design of semi-bionic method

关键词:  栀子柏皮汤  半仿生提取法  均匀设计  综合指标

Key words:  Zhizibaipi decoction  semi-bionic extraction method  uniform design  ≤1 000 u of molecular weight  extracted material  composite index

摘要:

[目的]优选栀子柏皮汤半仿生提取工艺条件。[方法]采用均匀设计法,在方药规格、煎提温度、煎提用水量、滤过、浓缩等条件相同的情况下,以分子量≤1 000 u提取物、栀子苷、小檗碱、甘草次酸、总生物碱为指标,综合评判,优选该方药半仿生提取的工艺条件。[结果]3煎用水的pH值依次为2.031 1、7.495 5、8.419 3,3煎总计时间为4.016 2 h。[结论]结合实际生产,确定3煎用水的pH值依次为2.0、7.5、8.5;提取时间依次为2、1、1 h。

Abstract:

[Objective] To optimize the semi-bionic extraction condition for Zhizi Baipi decoction.[Methods] Using the uniform design and taking the extracted material ≤1 000 u of molecular weight,Geniposide,berberine,glycyrrhetinic acid and total alkaloids as the index and under the same condition of the prescription specifications,the temperature of decocting and extracting,the used water volume,the filtration and concentration method,the art condition for semi-bionic extraction of this prescription was analyzed comprehensively and optimazed.[Results] The pH value of the three times of decocting water was 2.031 1,7.495 5 and 8.419 3.The total boiling time was 4.016 2 h.[Conclusion] In combination with actual production it was determined that the pH value for the three times of decocting water was 2.0,7.5,8.5 in tern and the extracting course was 2 h,1 h and 1 h respectively.