| 摘要: |
| [目的]建立补骨脂抗氧化活性指纹图谱,评价不同产地补骨脂药材的抗氧化活性和明确抗氧化活性成分的贡献率。[方法]采用高效液相色谱-二极管阵列检测器-1,1-二苯基-2-苦基肼(HPLC-DAD-DPPH)方法。[结果]建立了补骨脂抗氧化活性指纹图谱,确定了补骨脂酚为最主要的抗氧化活性成分。[结论]抗氧化活性指纹图谱可用于补骨脂质量评价,为全面提高中药质量控制水平提供了新方法。 |
| 关键词: 补骨脂 抗氧化活性指纹图谱 高效液相色谱-二极管阵列检测器-1,1-二苯基-2-苦基肼 |
| DOI：10.11656/j.issn.1672-1519.2011.02.27 |
| 分类号: |
| 基金项目:教育部科学技术重点项目(210013);国家自然科学基金面上项目(81001632);中国博士后基金(20100480657);高等学校博士学科点专项科研基金(20101210120007) |
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| Research on antioxidant activity fingerprint of Psoralea corylifolia L |
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CHANG Yan-xu, ZHU Zi-wei, LI Jin
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Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish the antioxidant activity fingerprint and to evaluate the antioxidant activity and investigate the contribution of individual constituents to antioxidant activity in Psoralea corylifolia L.[Methods] The HPLC-DAD-DPPH method was employed for that aim.[Results] A HPLC-DAD-DPPH method was optimized to establish antioxidant activity fingerprint of Psoralea corylifolia L.It was found that bakuchiol had the highest contribution of individual constituents to the antioxidant activity of Psoralea corylifolia L.[Conclusion] The antioxidant activity fingerprints is suitable for quality control of Psoralea corylifolia L.This study can clearly demonstrate that the activity fingerprint is a meaningful method to comprehensively improve the quality control of TCM. |
| Key words: Psoralea corylifolia L antioxidant activity fingerprint HPLC-DAD-DPPH |
