针刺对高脂大鼠主动脉脂联素受体1和2蛋白表达的影响

Influence of acupuncture on expression of aorta Adipo R 1 and 2 in hyperlipaemia rats

关键词:  脂联素受体  高脂  主动脉  针刺

Key words:  adiponectin receptor  hyperlipaemia  aorta  acupuncture

摘要:

[目的]探讨高脂饮食对SD大鼠主动脉脂联素信号的影响以及针刺对脂联素信号的调节。[方法]刚断乳的SD大鼠高脂喂养12周后检测血脂、髓过氧化物酶(MPO)和一氧化氮(NO),根据体质量随机分为模型组和针刺组,干预4周后用酶联免疫吸附实验(ELISA)法检测血清脂联素(APN),用Western blotting法检测主动脉脂联素受体(AdipoR)1和2蛋白表达,设普食对照组。[结果]高脂饮食12周后SD大鼠甘油三酯(TG)、胆固醇(TC)、MPO明显升高,NO明显降低;干预4周后模型组大鼠血清APN、主动脉AdipoR1/2蛋白含量均明显低于对照组,针刺组血清APN水平和主动脉AdipoR2含量明显增加,AdipoR1呈上升趋势。[结论]通过针刺能够增加血清APN水平和主动脉AdipoR蛋白,调整主动脉APN信号的转导,Adipo R2在其中发挥了重要作用。

Abstract:

[Objective] To explore the influence of high fat diet on aorta adiponectin signal and the regulatory effect of acupuncture on it in SD rats.[Methods] The SD rats were fed with high fat diet for 12 weeks in just delactated rats and then measured the blood lipid,MPO and NO.The rats were randomly divided into model group and acupuncture group according to the body weight.After intervention for 4 weeks serum APN was detected with ELISA and the expression of Adipo R 1 and 2 with Western blotting method.The rats feeding with common food were served as control.[Results] The levels of TG,TC,MPO were obviously increased and NO obviously decreased after feeding high fat diet for 12 weeks in SD rats.After intervention for 4 weeks,the serum adiponectin,protein content of Adipo R 1 and 2 of model group were distinctly lower than control group.The content of serum adiponectin and aorta Adipo R 2 were obviously increased,the level of Adipo R 1 had a rising tendency.[Conclusion] The hyperlipaemia rats feeding by high fat diet showed signal transduction disorder of aorta adiponectin.Acupuncture can regulate it by increasing serum APN and the level of aorta Adipo R 2.The Adipo R 2 plays a crucial role.