痹祺胶囊对实验性骨关节炎大鼠NO、HYP的影响

Effects of Biqi capsule on NO and HYP levels in rats with Osteoarthritis

关键词:  骨关节炎  痹祺胶囊  一氧化氮  羟脯氨酸

Key words:  osteoarthritis  Biqi capsule  NO  HYP

摘要:

[目的]观察痹祺胶囊对实验性大鼠膝骨关节炎(OA)一氧化氮(NO)、羟脯氨酸(HYP)的影响。[方法]选Wistar大鼠,木瓜蛋白酶膝关节注射造模,痹祺胶囊灌胃治疗8周,测定血清NO、HYP和尿HYP的含量。[结果]模型组造模后第1天尿HYP明显升高[(17.07±4.10)mg/L],第15天[(9.92±3.09)mg/L]和第64天[(13.77±1.32)mg/L]与正常组无明显差异。模型组大鼠血清NO含量明显升高(22.64μmol/L);痹祺胶囊高、中治疗组能明显降低大鼠血清NO含量(18.24μmol/L和19.14μmol/L)。[结论]痹祺胶囊具有降低OA大鼠血清NO的含量,减轻由NO介导的对关节软骨的损伤作用。

Abstract:

[Objective] To observe the effect of Biqi capsule on the Nitric oxide (NO) and hydroxyproline (HYP) in rats with osteoarthritis.[Methods] Wistar rats were used.Papain was injected into their knee joints to establish the animal model of osteoarthritis.They were treated with Biqi capsule through intragastric administration for 8 weeks.The contents of NO and HYP in serum and HYP in urine were detected.[Results] The contents of HYP in urine were obviously increased in model group in the first day [ (17.07±4.10) mg/L,P<0.05],and were normal in the fifteenth day and sixty-fourth day [ (9.92±3.09) mg/L, (13.77±1.32) mg/L,P>0.05].The contents of NO in serum were obviously increased (22.64 μmol/L,P<0.01) in model group.In treatment group the contents of NO in serum were decreased by Biqi capsule,1.296 g/kg and 0.648 g/kg (18.24 μmol/L and 19.14 μmol/L,P<0.01).[Conclusion] Biqi Capsule can decrease serum contents of NO in OA rats,and ameliorate the NO-mediated damage of the cartilage in joint.