| 摘要: |
| [目的]研究化瘀通脉方在大鼠体内的动力学特征及其不同配伍对组方中有效成分在体内吸收、分布和代谢的影响。[方法]采用Irregular-HC18(250 mm×4.6 mm,5μm)色谱柱,流动相甲醇-1%冰醋酸(40:60),柱温30℃,波长280 nm,测定灌胃化瘀通脉方及其不同配伍组方后的大鼠血浆和组织中的药物浓度,Kinetica 5.0软件对数据进行统计分析。[结果]建立了同时测定大鼠血浆及组织样品中丹参素、丹酚酸B、原儿茶醛和阿魏酸四种成分的高效液相色谱(HPLC)法,该方法的精密度、稳定性及回收率等均符合要求,线性关系良好。[结论]配伍对化瘀通脉方中丹参素在大鼠体内吸收、分布和代谢均具有积极的影响。 |
| 关键词: 化瘀通脉方 丹参 配伍 药动学 组织分布 |
| DOI：10.11656/j.issn.1672-1519.2011.03.29 |
| 分类号: |
| 基金项目:国家自然基金面上项目(30672664) |
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| Influence of compatibility on pharmacokinetic effects of Danshen in Huayu Tongmai decoction in rats |
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WANG Ping1, WANG Yang1,2, LI Jin2
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1.Tianjin University of TCM, Tianjin 300193, China;2.The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the pharmacokinetics of Huayu Tongmai prescription and the effect of its different compatibility on the absorption,distribution and metabolism of the effective components in vivo in rats.[Methods] The plasma and tissue drug levels of rat after intragastric administration of Huayu Tongmai prescription were determined and its different compatibility by HPLC.Chromatographic separation was achieved on a reversed-phase Irregular HC18 column (250 mm×4.6 mm,5 μm) at 30℃,with the mobile phase of methanol-1% acetic acid (40:60).The eluent was detected at 280 nm.The results were analyzed by Kinetica 5.0 software.[Results] The method for simultaneous determination of Danshensu,salvianolic acid B,protocatechuic aldehyde and ferulaic acid in rat plasma and tissue was established by HPLC.The stability,precision and recoveries with low,middle,high concentrations were satisfied.The linear ranges were all good.[Conclusion] Compatibility has the active effect on Danshen in Huayu Tongmai prescription. |
| Key words: Huayu Tongmai prescription Danshen compatibility pharmacokinetics tissue distribution |
