十二指肠胃食管反流动物模型的构建

Establishmentement of animal model with duodenum-stomach- esophageal reflux

关键词:  食管炎  十二指肠胃食管反流  模型  大鼠

Key words:  esophagitis  duodenum-stomach-esophageal reflux  model  rat

摘要:

[目的] 改进“四壁四针”间断吻合术式,完善十二指肠胃食管反流大鼠模型的构建。[方法] 空白组不作特殊处理,对照组和模型组都采用“四壁四针”间断吻合术式。对照组造模5 d后取材,模型组10 d后取材。 [结果] 模型组大鼠成活率(83.4%)较对照组(64.8%)差异有统计学意义(P<0.05),模型组大鼠食管下端黏膜肉眼表现较对照组差异有统计学意义(P<0.05),模型组大鼠食管下段病理改变与对照组差异有统计学意义(P<0.05).[结论] 改进术式的模型在增加存活率的基础上成功的诱发了反流性食管炎及Barrett食管。

Abstract:

[Objective] To improve the operation fashion of interrupted suture of “four walls and four stitches” and improve the establishment of animal models of deodenum-stomach-esophageal reflux. [Methods] The blank group(A) accepted no any special treatment while both the control(B) and the model(C) group received “four walls and four stitches” interrupted suture. Materials were obtained 5 days after modeling in group B while 10 days after that in C group. [Results] The survival rate of model group(83.4%) was significantly higher than that of control group(64.8%) (P<0.05). Both macroscopic characteristics and pathological changes of lower esophagus mucosa of rats in model group was significantly different from that of rats in control group (P<0.05). [Conclusion] The modified operating fashion in modeling can delay the survival rate and successfully induce reflex esophagitis and Barrett's esophagus.