| 摘要: |
| [目的]研究脑缺血不同时点大鼠海马神经元内游离Ca2+浓度[Ca2+]i及醒脑开窍针刺对其影响,探讨醒脑开窍针刺法早期干预对脑缺血的治疗作用。[方法]建立大脑中动脉所致局灶性脑缺血(MCAO)模型,采用醒脑开窍针刺法治疗,采用激光扫描共聚焦显微技术动态观察脑组织海马CA1区锥体细胞[Ca2+]i在不同时间点的变化。[结果]与正常组大鼠相比,模型组大鼠脑组织海马CA1区锥体细胞内游离[Ca2+]i(以细胞内Ca2+相对荧光强度表示)在局灶性脑缺血1 h时升高,持续升高至缺血24 h时(P<0.05)。假手术组与正常组相比[,Ca2+]i变化差异无统计学意义(P>0.05)。相应时间点非穴位针刺组与模型组[Ca2+]i比较,无统计学意义(P>0.05)。在相应时间点,醒脑开窍针刺组[Ca2+]i低于模型组(P<0.01)。[结论]急性局灶性脑缺血后大鼠海马神经细胞[Ca2+]i随缺血时间的延长而持续升高,提示细胞内钙超载;醒脑开窍针刺组能有效调节缺血区的[Ca2+]i,提示在缺血后针刺治疗效果越早越好,为临床及早应用针刺治疗缺血性脑血管病提供了理论依据。 |
| 关键词: “醒脑开窍”针刺法 局灶性脑缺血 海马CA1神经细胞 [Ca2+]i 钙超载 激光扫描共聚焦显微镜 |
| DOI：10.11656/j.issn.1672-1519.2012.02.13 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(30873304) |
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| Influence of acupuncture on [Ca2+]i in hippocampus neuron of rats with focal cerebral ischemia |
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YANG Sha, SHEN Yan, SUN Yun-hao, WANG Shu
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Acupuncture Institute of the First Hospital Affiliated of Tianjin University of TCM, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the dynamic changes of the [Ca2+]i in hippocampus neurons of rats with focal cerebral ischemia and the effect of "XNKQ" acupuncture to explore the the rapeutic action of early intervention with "XNKQ" acupuncture.[Methods] MCAO was established in rats.They were treated with "XNKQ" acupuncture method.At different time point the dynamic changes of the [Ca2+]i in hippocampus CA1 neurons were examined with confocal laser scanning microscope.[Results] Compared with the control group,the two neuron [Ca2+]i of hippocampus CA1 regions of model group began to increase after ischemia for 1h,and sustained to elevate until 24 h(P<0.05),while the sham operated group had no difference(P>0.05).At the same time point,there was no difference of [Ca2+]i between non-acupoint group and model group(P>0.05),while the "XNKQ" acupuncture group was significantly lower than that of the model group(P<0.05,P<0.01).[Conclusion] The [Ca2+]i in hippocampus CA1 neuron of rats is increased persistently during acute focal cerebralis chemia,causing the calcium overloading in cells.The study results also suggest that "XNKQ" acupuncture method can regulate the[Ca2+]i in the focal cerebral is chemia,and the earlier the better.All of these offer the evidence to use acupuncture at early time in clinic. |
| Key words: “XNKQ” acupuncture method focal cerebral ischemia hippocampus CA1 neuron [Ca2+]i calcium overloading confocal |
