补中益气汤对甲减肾损害模型大鼠肾脏功能、形态及VEGF表达的影响

Effect of Buzhong Yiqi decoction on the kidney function,morphology and VEGF expression in rats with hypothyroid kidney damages

关键词:  甲状腺功能减退症  肾损害  血管内皮生长因子  实验研究

Key words:  hypothyroidism  kidney damage  vascular endothelial growth factor  experimental research

摘要:

[目的]拟观察甲状腺功能减退时肾脏功能、形态及血管内皮细胞生长因子(VEGF)表达变化及补中益气汤对其的影响。[方法]将大鼠按随机数字表法随机分组,56 d后测定血清甲状腺功能[总三碘甲状腺原氨酸(TT₃)、总甲状腺素(TT₄)及促甲状腺激素(TSH)]、血清肌酐(SCr)、尿素氮(月哉晕)水平；光镜下观察肾脏形态,以免疫组化检测VEGF表达水平。[结果]1)与正常对照组相比左旋甲状腺素(L-T4)组及模型组血清BUN均增高(P<0.05),SCr也均增高(P<0.05)。圆)肾脏VEGF光密度:与正常对照组相比各组均无明显差异,与模型组相比只有补中益气汤组光密度明显升高(孕约园援园员)。3)PAS染色比较:处理21 d时,模型组呈轻度系膜增生,用药56 d后模型组有中度增生,有部分毛细血管扩张,两用药组以补中益气组增生最轻,L-T4组增生较模型组明显减轻,仍可见毛细血管扩张。[结论]补中益气汤能够促进VEGF分泌,VEGF升高对甲减肾损害具有保护作用。

Abstract:

[Objective] To study effect of Buzhong Yiqi decoction on the kidney function,morphology and VEGF expression in rats with hypothyroid kidney damages.[Methods] The rats were divided randomly into experiment group and normal group.56 days later we detected the level of serum TT₃,TT₄,TSH,SCr and BUN,observed the morphologic change of the kidney and the VEGF expression.[Results] 1)Compared with normal control group,the level of serum BUN(P<0.05) and SCr(P<0.05) in L-T4 group and model increased.2)About VEGF optical density,compared with normal control group,each group had no obvious difference.Compared with model group,Buzhong Yiqi decoction group increased obviously(P<0.01).3)About PAS staining,on the 21 days of treatment,model group displayed slightly hyperplasia;after 56 days treatment,model group displayed medium hyperplasia,the hyperplasia in Buzhong Yiqi decoction group was most slight among two treatment groups.[Conclusion] The conceivable mechanism of hypothyroid kidney damages is the loss of the glomerular capillaries.Buzhong Yiqi decoction can increase the endocrine function of VEGF and the rising VEGF in kidney plays a protective role for hypothyroidisn.