| 摘要: |
| [目的] 探讨PESV对K562细胞PI3K/Akt信号蛋白及凋亡调控因子Bcl-2和Bad表达的影响。[方法] 将体外培养K562细胞,经PESV处理不同时间后,流式细胞术检测细胞凋亡率,Western blot检测PI3K及p-Akt蛋白水平变化,实时荧光定量RT-PCR检测Bcl-2、Bad mRNA水平变化。[结果] 与对照组相比,PESV处理后K562细胞凋亡率显著增加,PI3K及 p-Akt表达降低,抗凋亡相关基因Bcl-2 mRNA表达降低,促凋亡基因Bad mRNA表达增加。[结论] PESV可能通过降低PI3K、Akt信号蛋白表达,调节Bcl-2和Bad表达,抑制K562细胞增殖,促进其凋亡。 |
| 关键词: PESV K562细胞系 凋亡 PI3K Akt Bcl-2 Bad |
| DOI：10.11656/j.issn.1672-1519.2012.03.24 |
| 分类号: |
| 基金项目:教育部博士点基金项目(20091210110004). |
|
| Study on PI3K/Akt messenger passage and apoptosis controlling in PESV-preconditioned K562 cells |
|
ZHANG Lei1, YANG Wen-hua1, YU Wen-jun2, HAO Zheng2, ZHANG Jia2
|
|
1.The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China;2.Tianjin University of TCM, Tianjin 300193, China
|
| Abstract: |
| [Objective] To investigate the effect of PESV on the expression of massage protein PI3K / Akt, apoptosis regulators Bcl-2 and Bad expression in K562 cells. [Methods] In vitro cultured K562 cells preconditioned with PESV for different times were determined for cell apoptosis rate with cytoflowmeter, the level of PI3K and p-AKT protein with Western blot and Bcl-2, Bad mRNA level by realtime fluorescence quantitative RT-PCR. [Results] Compared with the control group, the apoptosis rate of PESV treated K562 cells was significantly increaseds, the expression of PI3K and p-Akt and anti-apoptosis-related gene Bcl-2 mRNA was decreased; the expression of pro-apoptotic gene Bad mRNA was increased. [Conclusion] PESV may regulate the expression of Bcl-2 and Bad, inhibit the proliferation of K562 cells and promote their apoptosis by reducing the expression of PI3K and Akt signaling protein. |
| Key words: PESV K562 cell lines apoptosis PI3K Akt Bcl-2 Bad |
