心肌微环境下丹酚酸B诱导MSCs 向心肌样细胞分化的研究

Study on differentiation of mesenchymal stem cells into cardiomyocyte-like cells induced by SalB in cardiac micro-environment

关键词:  骨髓间充质干细胞  心肌样细胞  丹酚酸B  心肌微环境

Key words:  mesenchymal stem cells  cardiomyocyte-like cells  SalB  cardiac micro-environment

摘要:

[目的] 复制心肌缺血-再灌注损伤模型,体外模拟心肌缺氧复氧微环境,观察丹酚酸B(SalB)诱导骨髓间充质干细胞(MSCs)向心肌样细胞分化的作用。[方法] 通过向MSCs培养体系中添加心肌细胞裂解液的方法,体外模拟心肌微环境。自成年大鼠骨髓中分离MSCs,设对照组(A组)、心肌细胞裂解液(TJ)组(B组)、SalB组(C组)、TJ+SalB组(D组).培养4周,观察细胞形态,并采用免疫荧光技术检测cTnT的表达。[结果] 除A组MSCs无明显的肌样细胞形成,各诱导组均有肌样细胞分化,D 组与C组相比,cTnT表达率明显增高,差异有统计学意义。[结论] 心肌细胞裂解液可以诱导MSCs向心肌样细胞分化;心肌微环境的建立有利于 SalB诱导 MSCs向心肌样细胞分化。

Abstract:

[Objective] To reproduce the model of myocardial ischemia-reperfusion injury to simulate cardiac micro-environment in vitro and observe the effect SalB in the process of mesenchymal stem cells(MSCs )differentiating into cardiomycyte-like cells. [Methods] A myocardium micro environment was simulated in vitro by adding myocardial cell lysate to MSCs culture system. MSCs was obtained from adult rats' bone marrow, and divided into four groups: the control group (group A), myocardio cell lysate group (TJ, group B), SalB group (group C) and TJ+SalB group (group D). They were cultivated for four weeks, and then the morphology was observed and the cTnT expression was detected by immune fluorescence detection technology. [Results] Each induced group had myocardial-like cell differentiation except group A. Compared with group C the cTnT expression in Group D was the most obvious with a statistical significance between them. [Conclusion] Myocardial cell lysate can induce MSCs differentiating into cardiomyocyte-like cells. Cardiac micro-environment can contribute SalB to induce differentiation of MSCs into cardiomyocyte-like cells.