更年平调液对更年期大鼠心肌细胞保护及 Bcl-2、Bax表达的影响

Effect of Gengnian Pingtiao liquid on expression of Bcl-2 and Bax in myocardial cell of rats with climacteric

关键词:  更年平调液  心肌  更年期大鼠  Bcl-2  Bax  凋亡  免疫组织化学

Key words:  Gengnian Pingtiao liquid  cardiac muscle  menopause rats  Bcl-2  Bax  apoptosis  immunohistochemistry

摘要:

[目的] 探讨更年平调液对更年期大鼠心肌细胞保护作用的机制.[方法] 选用鼠龄为11～15个月的自然更年期雌性SD大鼠,20只,随机分为2组,即更年平调液组(P组)、更年期对照组(M组).另选鼠龄为4～6个月的SD大鼠,10只,为青年对照组(Y组).药物干预第45天,采用免疫组织化学方法检测各组心肌组织中Bcl-2、Bax的表达,利用计算机图像分析技术测量各组心肌组织中Bcl-2、Bax的表达水平. [结果] 更年平调液组Bcl-2、Bax的表达较更年期对照组差异有统计学意义(P < 0.01),更年平调液组与青年对照组比较Bcl-2、Bax的表达差异无统计学意义 (P > 0.05).[结论] 更年平调液能抑制心肌细胞凋亡而对心肌细胞起着重要的保护作用.

Abstract:

[Objective] To explore of protective effects of Gengnian Pingtiao liquid on myocardial cell of rats with Climacteric. [Methods] The 20 SD rats of 11 to 15 months old with the natural climacteric were randomly divided into two groups: Gengnian Pingtiao liquid group (group P), menopause control group (group M). Another 10 SD rats, 4~6 month old were taken for young control group (group Y). After experiment for 45 days, the average optical density(OD) and the rate of positive area of expression of Bcl-2 and Bax were analyzed by image analysis with HPIAS-2000 analysis system. [Results] The expression of Bcl-2 in group P was significantly higher than that in group M (P < 0.01). No significant difference of expression of Bcl-2 was found between the group P and that in group Y (P ＞ 0.05). The expression of Bax in group P was significantly lower than that in group M (P < 0.01). No significant difference was found in the expression of Bax between group P and that in group Y (P ＞ 0.05). [Conclusion] Gengnian Pingtiao liquid can inhibit the myocardial cell apoptosis and plays an important protective role for myocardial cell.