疏肝健脾方对腹泻型肠易激综合征大鼠血浆及结肠组织5-HT、SP、VIP的影响

Effects of Shugan Jianpi prescription on 5-HT, SP and VIP content in the plasma and colon of D-IBS rat

关键词:  疏肝健脾方  腹泻型肠易激综合征  胃肠激素  5-羟色胺  P物质  血管活性肠肽

Key words:  Shugan Jianpi prescription  the diarrhea predominant irritable bowel syndrome  intestinal hormones  5-HT  SP  VIP

摘要:

[目的] 观察疏肝健脾方对腹泻型肠易激综合征大鼠血浆及结肠组织5-羟色胺(5-HT)、P物质(SP)和血管活性肠肽(VIP)的影响。[方法] 将60只SD大鼠按随机数字表法随机分为正常组、模型组、得舒特组、疏肝健脾方低、中、高剂量组, 使用乙酸灌肠加束缚的方法建立腹泻型肠易激综合征(D-IBS)大鼠模型, 应用免疫组化法检测5-HT、SP及VIP. [结果] 疏肝健脾方可以明显降低大鼠的粪便含水量(P<0.05);疏肝健脾方中、高剂量组腹部撤退反射(AWR)评分在不同扩张容量下均较模型组明显降低(P<0.05);疏肝健脾方可以降低大鼠血浆中5-HT和结肠组织中SP的含量, 而血浆中SP、VIP及结肠组织中VIP含量无显着变化。[结论] 疏肝健脾方可以改善大鼠的腹泻及内脏的高敏感性, 并对5-HT及SP有调节作用, 提示疏肝健脾方可以有效治疗D-IBS, 并与调节胃肠激素有关。

Abstract:

[Objective] To observe the effect of Shugan Jianpi prescription (SGJP) on the concentration of 5-HT, SP and VIP content in the plasma and colon of D-IBS rat. [Methods] Sixty SD male rats were randomly divided into six groups with ten rats each: normal group, D-IBS model group, pinaverium bromide group and SGJP low, middle, high dosage group. D-IBS rat model was induced by intracolonic instillation of acetic acid and restraint stress and immunohistochemistry technology was employed to test the 5-HT, SP and VIP content in the plasma and colon of rat. [Results] The defecation moisture was significantly reduced by the SGJP prescription (P<0.05); the AWR scores of the D-IBS rats under different expansion volume were reduced after treated with the middle and high dosage of SGJP prescription (P<0.05); the content of plasma 5-HT and colon SP of D-IBS rats was reduced after administration with SGJP prescription, but plasma SP, VIP and colon VIP were not influenced. [Conclusion] The SGJP prescription could improve the diarrhea and visceral hypersensitivity and regulate the content of 5-HT and SP, which shows that the SGJP prescription could efficiently treat D-IBS by regulating gut hormones.