大鼠MSCs的体外分离、培养及鉴定的实验研究

Experimental study on isolation, culture and identification of MSCs in rats

关键词:  MSCs  密度梯度离心法  体外分离  鉴定

Key words:  Bone marrow mesenchymal stem cell  density gradient centrifugation method  isolation in vitro  identification

摘要:

[目的] 体外分离、 培养及鉴定大鼠骨髓间充质干细胞(MSCs).[方法] 密度梯度离心法体外分离、 培养7 周龄 SD 大鼠MSCs, 利用差速贴壁原理纯化MSCs, 并通过形态学观察、 细胞表面抗原标志物、 多细胞系诱导分化对其进行鉴定。[结果] 第3代(P3代)不表达抗原 CD₃₄, 表达抗原CD₄₄, 符合MSCs表面标志物特征。[结论] 密度梯度离心法能够建立稳定的MSCs体外分离培养体系。

Abstract:

[Objective] To isolate, culture and purify bone marrow mesenchymal stem cells(MSCs). [Methods] MSCs from the rat bone marrow was obtained and purified in vitro by the method of gradient centrifugation combining with adherence culture. They were assess by immunocytochemistry. [Results] The samples of MSCs of third passage could express CD₄₄, but not CD₃₄, according to the characteristics of the MSCs. [Conclusion] The method of density gradient centrifugation can establish a stable system to separate and culture MSCs in vitro.