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| 高效液相色谱法同时测定青蒿绿原酸、隐绿原酸、东莨菪内酯含量 |
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孙玉刚1,2, 秦续文3, 张玲1, 李晋1, 萧伟3, 王振中3, 成志东3, 常艳旭1,3,2
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津中医药大学中医药研究院, 中药化学与分析重点实验室, 天津 300193;3.江苏康缘药业股份有限公司博士后工作站, 连云港 222001
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| 摘要: |
| [目的] 通过高效液相色谱法测定青蒿中绿原酸、隐绿原酸和东莨菪内酯的含量,并对人工规范化种植的青蒿中绿原酸、隐绿原酸和东莨菪内酯进行测定, 为青蒿药材质量评价提供分析方法。[方法] Agilent Zorbax SB-C18反相色谱柱(4.6 mm×250 mm, 5 μm), 乙腈-0.1%甲酸水为流动相, 波长325 nm, 柱温30℃, 流速1.0 mL/min, 进样量10 μL.[结果] 绿原酸、隐绿原酸、东莨菪内酯在范围内呈现良好的线性关系, 方法学验证结果均符合相关要求。[结论] 该方法简便可行, 重复性好, 专属性强, 可用于青蒿药材的质量控制。 |
| 关键词: 青蒿 高效液相色谱法 绿原酸 |
| DOI：10.11656/j.issn.1672-1519.2012.05.24 |
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| 基金项目:“十一五”国家重大科技专项“重大新药”创制项目(2010ZX09502-005);江苏省博士聚集计划;天津市高等学校科技发展基金项目(20040323). |
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| Simultaneous determination of chlorogenic acid, cryptochlorogenic acid and scopoletin in Artemisia Annua L. by HPLC |
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SUN Yu-gang1,2, QIN Xu-wen3, ZHANG Ling1, LI Jin1, XIAO Wei3, WANG Zhen-zhong3, CHENG Zhi-dong3, CHANG Yan-xu1,3,2
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1.Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China;2.Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China;3.Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China
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| Abstract: |
| [Objective] A sensitive method of the determination of chlorogenic acid, cryptochlorogenic acid and scopoletin content in traditional Chinese medicine Artemisia annua L. was established by high performance liquid chromatography (HPLC). [Methods] The separation was achieved by use of C18 analytical column (4.6 mm×250 mm, 5 μm) at 30℃, while the injected sample volume was set to 10 μL. A constant flow rate of 1.0 mL/min was employed throughout the analysis. A binary gradient elution program of aqueous formic acid (0.1%, v/v) versus acetonitrile was selected for the quantitative analysis of chlorogenic acid, cryptochlorogenic acid and scopoletin. [Results] The HPLC method showed the satisfactory linearity, and the results of the method validation meeted the relevant requirements. The method had been successfully applied to the determination of chlorogenic acid, cryptochlorogenic acid and scopoletin content in Artemisia L. samples. [Conclusion] The established HPLC method could be used to evaluate the quality of Artemisia annua L. |
| Key words: Artemisia Annua L. HPLC chlorogenic acid |
