| 摘要: |
| [目的] 建立香砂养胃丸中香附的α-香附酮含量测定方法。[方法] 采用Agilent ZORBAX TC-C18(250 mm×4.6 mm, 5 μm)色谱柱;以甲醇-水(75:25)为流动相;流速为1.0 mL/min;检测波长为250 nm.[结果] α-香附酮在0.005 5～0.55 μg/mL范围内呈良好线性关系(r=0.999 9), 平均回收率为102.36%, RSD为1.75%(n=6).[结论] 该法简便、结果准确、重复性好, 可用于香砂养胃丸的质量控制。 |
| 关键词: 香砂养胃丸 α-香附酮 含量测定 高效液相色谱法 |
| DOI：10.11656/j.issn.1672-1519.2012.05.25 |
| 分类号: |
| 基金项目: |
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| Determination of α-Cyperone in Xiangsha Yangwei pills by HPLC |
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TU Ying
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Department of Pharmacy, Tianjin Chest Hospital, Tianjin 300051, China
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| Abstract: |
| [Objective] To develop an HPLC method for the determination of α-Cyperone in Xiangsha Yangwei pills. [Methods] Analyses were carried out on Agilent ZORBAX TC-C18 column(250 mm ×4.6 mm, 5μm) with methanol-water (75:25) as the mobile phase. The flow rate was 1.0 mL/min and the UV detection wavelength was set at 250 nm. [Results] The linearities of α-Cyperon were in the range of 0.005 5~0.55 μg/mL(r=0.999 9). The average recoveries of α-Cyperon were 102.36%.The RSD were 1.75% (n=6). [Conclusion] The method is simple, accurate, strongly specific and can be used for the quality control of Xiangsha Yangwei pills. |
| Key words: Xiangsha Yangwei pills α-Cyperone determination HPLC |
