火针对大鼠脊髓损伤后运动功能及BDNF表达的影响

Influence of fire needle on sport ability and expression of BDNF after spinal cord injury in rats

关键词:  脊髓损伤  火针  脑源性神经营养因子

Key words:  spinal cord injury  fire needle  brain-derived neurotrophic factor

摘要:

[目的] 探讨火针对脊髓损伤后运动功能及脑源性神经营养因子(BDNF)表达的影响。[方法] 将60只SD大鼠随机分为3组:假手术组、模型组和火针组。采用改良Allen法制作脊髓损伤动物模型。火针组术后即刻及其后每隔24 h火针针刺大鼠脊髓损伤部位上下端的棘突间隙,另两组相应时间点行相同抓握。于伤后1、3、5、7 d采用大鼠脊髓损伤后运动功能(BBB)评分标准进行不同时段的行为学评分并取材。通过免疫组化法观察各组大鼠脊髓损伤后BDNF表达的变化。[结果] BBB评分显示,火针组的各时间点评分均高于模型组,特别是在造模并干预7 d后效果更加显着(P<0.05).与假手术组相比,模型组和火针组的BDNF表达在各时间点均增加,火针组3、5、7 d的BDNF表达均高于模型组,差异具统计学意义(P<0.05).BBB评分与BDNF表达量相关性分析显示两者呈高度相关。[结论] 火针可促进大鼠脊髓损伤后运动功能恢复,其机制与促进BDNF表达有关。

Abstract:

[Objective] To explore the influence of fire needle on sports ability and the expression of brain-derived neurotrophic factor(BDNF) after spinal cord injury. [Methods] Sixty SD rats were randomly divided into sham operation, model and fire-needle groups. The modified Allen's method was adopted to prepare the spinal cord injured model. After the operation, fire needle were applied to the upper and lower spinous process intervals of the injured segment in rats immediately and once every 24 h afterwards in fire needle group, and with the same grasp of rats in the two left groups at corresponding time point. The BBB scoring standard was used to evaluate the behavioral score and the specimens were taken in different stages after 1, 3, 5 and 7 d of injury. The changes of the expression of BDNF after spinal cord injury in rats of each group were detected by immunohistochemical method. [Results] According to the BBB scores, all the scores of fire needle group are higher than those of model group at each time point, especially 7 days after modeling and intervention with more obvious effects (P<0.05). Compared with sham operation group, the expressions of BDNF in the model group and fire needle group were significantly increased at each time point, and the expressions of BDNF in fire needle group were higher than those in model group in 3, 5 and 7 d. The difference was statistically significant (P<0.05). The correlation analysis between BBB scores and the expressions of BDNF showed that they were highly related. [Conclusion] Fire needle therapy can promote the recovery of sports ability after spinal cord injury in rats, and the mechanism is related to the expression of BDNF.