生肌象皮膏对2型糖尿病大鼠溃疡肉芽组织的胰岛素样生长因子-1及受体的影响

Effect of Shengji Xiangpi Gao on insulin-like growth factor-1 and its receptor in wound granulation tissue in rats with diabetic ulcer

关键词:  生肌象皮膏  糖尿病溃疡  胰岛素样生长因子-1  胰岛素样生长因子-1受体

Key words:  Shengji Xiangpi Gao  diabetic ulcer  insulin-like growth factor-1  insulin-like growth factor-1 receptor

摘要:

[目的] 对糖尿病大鼠溃疡创面肉芽组织的胰岛素样生长因子-1(IGF-1)及受体(IGF-1R)含量的动态观察,探讨生肌象皮膏促进糖尿病大鼠溃疡愈合的机制。[方法] 采用高脂饲料加链脉佐菌素(STZ)尾静脉注射制备2型糖尿病动物模型,模型动物于背部打孔,造成溃疡模型。糖尿病溃疡Wistar大鼠动物按随机数字表法随机分为糖尿病空白对照组、凡士林组、生肌象皮膏组。分别于1、7、14、21 d每组处死动物10只,手术取新鲜肉芽组织160 例,采用实时荧光定量PCR法检测IGF-1及受体的含量。[结果] 生肌象皮膏组、凡士林组、糖尿病对照组IGF-1的表达于7 d增加,第14天达到高峰,在21 d各组的IGF-1的含量明显低于空白组。在第7、14天凡士林组、糖尿病对照组与空白组比较P<0.05.生肌象皮膏组与空白组比较具有增加的趋势。在21 d凡士林组、糖尿病对照组、生肌象皮膏组IGF-1含量明显下降,与空白组比较P<0.05,糖尿病各组间比较各时间点无统计学意义。生肌象皮膏组、凡士林组、糖尿病对照组IGF-1R的分泌在各时间段均低于空白组,且呈递减趋势。第7、14、21天空白组、生肌象皮膏组与糖尿病对照组比较有显着性差异。第14、21天生肌象皮膏组与凡士林组比较有显着性差异。[结论] IGF-1在糖尿病溃疡早期、中期含量增加,而糖尿病溃疡后期含量减少。生肌象皮膏不能促进糖尿病溃疡肉芽组织中的胰岛素样生长因子的表达,却能增加胰岛素样生长因子受体的表达。

Abstract:

[Objective] To observe the expression of insulin-like growth factor-1 (IGF-1) and its receptor (IGF-IR) content in the wound granulation tissue of rats with diabetic ulcer, explore the mechanism of Shengji Xiangpi Gao for promoting ulcer healing. [Methods] Using high fat diet plus tail vein injection with STZ preparation a model of type 2 diabetes was induced and perforating a hole in the back to make ulcer model. The rats according to the level of blood glucose and body weight randomly divided into blank group, vaseline group, Shengji Xiangpi Gao group. In 1, 7, 14, 21 d, 10 rats in each group were sacrificed, and fresh granulation tissue was obtained in 160 cases. Using real-time fluorescence quantitative PCR assay, the content of IGF-1 and receptor were determined [Results] The expression of IGF-1 increased at 7 d in Shengji Xiangpi Gao group, vaseline group, diabetic control group, reaching the peak at 14 days. In 21 d, the amount of IGF-1 in each group was obviously lower than that of blank group. In 7, 14 d, compared with vaseline group, diabetic control group it was P<0.05 in control group. Compared with blank group, Shengji Xiangpi Gao group had a increasing trend. In 21 d, compared with control group, IGF-1 decreased significantly in vaseline group, diabetic control group, Shengji Xiangpi Gao group (P<0.05). IGF-1R secretion in Shengji Xiangpi Gao group, vaseline group, diabetic control group in each time period was lower than the control group, and showed a decreasing tendency. In 7, 14, 21 d compared with control group, blank group and Shengji Xiangpi Gao group had significant difference. In 14, 21 d there was significant difference between Shengji Xiangpi Gao group and vaseline group. [Conclusion] IGF-1 increases in early and, middle period of diabetic ulcers, but decreases in the late. Shengji Xiangpi Gao can not promote the expression of IGF in diabetic ulcer granulation tissue, but can increase the expression of IGF receptor.