热毒清对急性肝损伤大鼠内毒素血症的影响

Effect of Reduqing on endotoxemia in rats with acute liver injury

关键词:  热毒清  急性肝损伤  内毒素血症  菌群失调  肝衰竭

Key words:  Reduqing  acute liver injury  endotoxemia  flora  liver failure

摘要:

[目的] 探讨中药制剂热毒清对急性肝损伤大鼠内毒素血症的影响。[方法] 将实验大鼠分成4组(正常对照组、急性肝损伤组、治疗组、预防加治疗组),以D-氨基半乳糖制备急性肝损伤模型,并对治疗组及预防加治疗组以热毒清处理,动态观察4组血浆内毒素(ET)、过氧化脂质(LPO)、纤维结合素(Fn)水平及肝脏病理变化,检测肠道细菌并鉴定。[结果] 治疗组各时相ET水平均低于急性肝损伤组,预防加治疗组在24、72 h ET低于治疗组;治疗组LPO水平在各时相均低于急性肝损伤组,预防加治疗组在24、36 h LPO低于治疗组;预防加治疗组比治疗组Fn提升更早(P<0.01);急性肝损伤组肝细胞损伤程度重的比例大于治疗组和预防加治疗组(P<0.05);预防加治疗组肠杆菌、肠球菌计数低于治疗组,而乳酸杆菌和双歧杆菌高于治疗组(P<0.01).[结论] 热毒清具有抗内毒素、抗脂质氧化和保护肝细胞作用,而且能调节肠道菌群失调,可作为治疗肝衰竭肠源性内毒素血症的方法之一。

Abstract:

[Objective] To investigate the effect of traditional Chinese medicine Reduqing on endotoxemia of acute liver injury in rats. [Methods] Rats were divided into four groups (normal control group, acute liver injury group, treatment group, prevention plus treatment group). We prepared the model of acute liver injury in rats with D- galactosamine. The treatment group and the prevention plus treatment group were treated by Reduqing. Plasma endotoxin (ET), lipid peroxide (LPO), fibronectin (Fn) levels and liver pathology of four groups were continuously observed, and intestinal bacterial culture were detected and identified. [Results] ET in treatment group at each time point was lower than the group of acute liver injury. ET in prevention plus treatment group was lower than treatment group at the 24, 72 hours. Levels of LPO in all treatment groups were lower than the group of acute phase of liver injury. LPO in prevention plus treatment group was lower than the treatment group at 24, 36 hours; Fn in prevention plus treatment group increased earlier than in the treatment group(P<0.01); in acute liver injury group proportion of serious damage in liver cells was higher than in the treatment group and the prevention plus treatment group(P<0.05); the content of enterobacter and enterococcu in the prevention plus treatment group was less than in the treatment group, but lactobacillus and bifidobacteri were higher than treatment group (P<0.01). [Conclusion] Reduqing can significantly improve the liver injury in rats after poisoning. Therefore, “Reduqing” possesses anti-endotoxin, anti-lipid peroxidation and protective effect of liver cells, can regulate the intestinal flora and can be used as one of method in treating intestinal endotoxemia in hepatic failure.