| 摘要: |
| [目的] 建立益肾颗粒中淫羊藿苷含量的测定方法。[方法] 采用高效液相色谱法(HPLC),色谱柱为美国Waters公司C18柱(250 mm×4.6 mm, 10 μm), 流动相为乙腈-0.1%磷酸溶液(28:72), 流速为1 mL/min,检测波长为270 nm, 柱温为室温。[结果] 淫羊藿苷在0.048 5~0.970 0 μg内与色谱峰面积线性关系良好(r=0.999 9), 平均回收率97.0%, RSD=0.7%(n=6).[结论] 该法简便、专属性、重复性好, 可用于益肾颗粒中淫羊藿苷的含量测定。 |
| 关键词: 益肾颗粒 淫羊藿苷 高效液相色谱法 |
| DOI：10.11656/j.issn.1672-1519.2013.06.15 |
| 分类号: |
| 基金项目:国家中医药管理局资助项目(200807047). |
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| Determination of icariin content in Yishen granules by HPLC |
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LIU Ya-qin, LI Feng-li, XUE Kun
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The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish a method to determine the content of icariin in Yishen granules. [Methods] Using the method of HPLC, the chromatographic column was the United States Waters C18 column (250 mm × 4.6 mm, 10 μm). The mobile phase was methyl cyanides-0.1% phosphoric acid solution (28:72). Velocity of flow rate was 1 mL/min. The detection wavelength was 270 nm, and column temperature was at room temperature. [Results] Icariin in 0.048 5~0.970 0 μg and chromatographic peak area had good linear relationship (r=0.999 9). The average recovery was 97%, RSD=0.7% (n=6). [Conclusion] The method is simple, specific, reproducible, and can be used for determination of icariin in Yishen granules. |
| Key words: Yishen granule icariin HPLC |
