蜂胶体外抑菌活性的研究

Observation on the bacteriostatic activity of propolis in vitro

关键词:  蜂胶  抑菌作用  活性研究

Key words:  propolis  antibacterial activity  study of activity

摘要:

[目的] 探讨蜂胶对6种常见皮肤致病菌苏云杆菌、枯草芽孢杆菌、大肠杆菌、藤黄微球菌、白色葡萄球菌、奇异变形杆菌的抑菌效果。[方法] 采用琼脂平板扩散法, 6种受试菌株分别接种到不同的板上, 每块板设置4种不同的浓度及对照组, 12 h后观察蜂胶对各致病菌的抑菌圈大小, 评价蜂胶对各致病菌的敏感程度及抑菌活性。[结果] 不同浓度下, 蜂胶对同一种受试菌株的敏感程度不同, 抑菌圈大小不同;随着浓度的减小, 抑菌圈逐渐减小, 抑菌活性减弱。在同一浓度下, 蜂胶浓度为10%~20%时, 蜂胶对各受试菌株均表现出较高的抑菌活性;蜂胶对各受试菌株抑菌的强弱顺序为:枯草芽孢杆菌> 苏云杆菌>藤黄微球菌>奇异变形杆菌>白色葡萄球菌>大肠杆菌。[结论] 蜂胶对6种常见致病菌有不同程度的抑菌作用, 对苏云杆菌、枯草芽孢杆菌有很强的抑菌作用, 为作为外用药等提供依据。

Abstract:

[Objective] To investigate the antibacteria activity of propolis on different concentration, Bacillus thuringiensis, Bacillus subtilis, Escherichia coli Micrococcus luteus, Streptococcus white, Proteus Mirabilis was used in the testing. [Methods] Each plate which smeared with culture mediums was designed with six tested bacterium, and four different concentrations of propolis and two contrasts was used for bacteriostatic test in each testing plate. The sensitive and antibacteria activity was tested by bacteriostatic ring which was measured and recorded in each group after 12 hours. [Results] It was found that the diameters(in mm) of bacteriostatic rings in each plate with different concentrations of propolis were respectively recorded as follows. In the same concentration, external use of Propolis has significant antibacteria activit, especiouly in the concentration of 10%～20%. Bacillus subtilis>Bacillus thuringiensis> Micrococcus luteus>Proteus. mirabilis>Streptococcus white>Escherichia coli. [Conclusion] It suggests that antibacterial activity of propolis against all tested bacterium weakens along with the concentration. In the same concentration, the antibacteria activity of propolis was very sensitive to the six tested bacterium in different degree, and the antibacteria activity of propolis to Bacillus thuringiensis as well as Bacillus subtilis was the best. The external use of antipyresis and detumescence can be provide by this experiment.