紫癜汤抑制ITP模型小鼠血小板破坏的作用机制研究

Mechanismof Zidian decoction for inhibiting platelet destruction in ITP in mouse

关键词:  紫癜汤  特发性血小板减少性紫癜  巨噬细胞  凋亡

Key words:  Zidian decoction  idiopathic thrombocytopenic purpura  macrophage  apoptosis

摘要:

[目的] 紫癜汤对特发性血小板减少性紫癜(ITP)模型小鼠脾脏巨噬细胞(MΦ)凋亡及微观结构的影响,探讨该方临床使用的作用机制.[方法] 将48只BALB/C小鼠随机分为4组:正常组、模型组、泼尼松组、紫癜汤组,除正常组外,其余各组采用免疫法腹腔注射豚鼠抗小鼠血小板血清(GP-APS)建立ITP小鼠模型,观察小鼠外周血小板、脾中巨噬细胞的微观结构及其凋亡情况.[结果] 治疗后,两药物组均能提高ITP小鼠血小板计数,与用药前比较差异有统计学意义;紫癜汤组酝椎的凋亡率提高,与模型组比较,疗效优于醋酸泼尼松(P<0.05);两药均能改善酝椎的微观结构,紫癜汤优于醋酸泼尼松.[结论] 紫癜汤通过改善ITP模型小鼠脾中巨噬细胞的微观结构及促进巨噬细胞的凋亡起到治疗作用.

Abstract:

[Objective] To observe the effects of Zidian decoction on apoptosis and microstructure of macrophage (MΦ) in spleen in ITP in mouse and to explore the mechanism of action of the prescription in the clinical use.[Methods] The 48 BALB/C mice were randomly divided into 4 groups: the normal group,model group,prednisone acetate group-Zidian decoction group.ITP was induced with intraperitoneal injection of GP-APS (guinea pig anti-mouse platelet serum) by immunoassay in mice.We observed the microscopic structure and apoptosis of peripheral platelet and spleen macrophages.[Results] After treatment-platelet count in the treatment group increased, there was a significant difference when compared with before treatment; after treatment, MΦ apoptosis rate was increased in Zidian decoction group compared with the model group(P<0.05) and the therapeutic effect was superior to prednisone.[Conclusion] Zidian decoction has therapeutic effect by improving the microstructure of spleen macrophages in ITP and promoting macrophage apoptosis in mice.