葛根-石膏药对不同配伍比例中有效成分含量变化研究

Change of effective contents in different proportion of compatibility of Radix Puerariae Lobatae-Gypsum Fibrosum couple

关键词:  葛根-石膏  配伍  葛根素  大豆苷  超高效液相色谱

Key words:  Radix Puerariae Lobatae-Gypsum Fibrosum  compatibility  puerarin  soybean saponin  UPLC

摘要:

[目的] 通过建立超高效液相色谱(UPLC)法测定葛根-石膏药对中葛根素和大豆苷的含量,并通过此法探讨有效成分随葛根石膏配比变化的溶出规律。[方法] 不同比例葛根石膏配伍,水煎提取,采用超高效液相色谱(UPLC)法,色谱柱: Acqioty UPLC C₁₈,2.1 mm×100 mm,1.7 μm,检测器:二极管阵列检测器,工作站:Empower 3,流动相:乙腈-甲醇-1.5‰磷酸水溶液梯度洗脱,检测波长:254 nm。[结果] 葛根配伍石膏,葛根素和大豆苷煎出量均比葛根单煎高。以葛根-石膏(1:2)时葛根素、大豆苷的煎出率最高。[结论] 说明葛根配伍石膏有利于葛根素和大豆苷的煎出,从而增加有效成分的溶出。

Abstract:

[Objective] To test the content of puerarin and soybean saponin in the drug couple of Radix Puerariae Lobatae and Gypsum Fibrosum using the established UPLC method and the elution rule of effective content as the change of the compatibility of Radix Puerariae Lobatae and Gypsum Fibrosum was explored. [Methods] Radix Puerariae Lobatae and Gypsum Fibrosum in different compatibility proportion were decocted with water. UPLC was used to determine the change in different group of Radix Puerariae Lobatae and Gypsum Fibrosum. Separation was performed on Acquity UPLC, C₁₈, 1.7 μm, 2.1 mm×100 mm column; detector: PDA; workstation: Waters Empower 3; the motile phase: acetonitrile-methanol-1.5‰ phosphoric acid-water gradient elution and the detection wavelength was set at 254 nm. [Results] Radix Puerariae Lobatae decocted together Gypsum Fibrosum could increase the elution content of puerarin and soybean saponin compared with Radix Puerariae Lobatae only. When the proportion was 1:2 (Radix Puerariae Lobatae-Gypsum Fibrosum) the elution amount of puerarin and soybean saponin was the most.[Conclusion] The compatibility of Radix Puerariae Lobatae with Gypsum Fibrosum is beneficial for decocting of puerarin and soybean saponin. It can improve the decocted quantity of effective substance evidently.