| 摘要: |
| [目的] 研究中药复方妇科灌肠Ⅱ号冻干粉对Caco-2细胞的影响。[方法] 利用噻唑蓝(MTT)法检测妇科灌肠Ⅱ号作用在Caco-2细胞的安全浓度。[结果] 当妇科灌肠Ⅱ号浓度高于0.1 g/L时,随着浓度的增加,细胞存活率呈下降趋势。[结论] 以Caco-2细胞作为新工具引入中医外导法的基础与安全性研究,可能成为一项有价值的探索。 |
| 关键词: 中医外导法 Caco-2细胞模型 妇科灌肠Ⅱ号 |
| DOI：10.11656/j.issn.1672-1519.2013.09.14 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(81072837) |
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| Study on the rectum absorption mechanism of the traditional Chinese medicine based on Caco-2 cell model |
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LI Pei-lin1, WANG Pan-pan2, ZHAO Zhi-mei1, LI Jie1, WANG Jian-ling1
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1.The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China;2.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the effects of Fuke Guanchang Ⅱ recipe on human colon carcinoma cell line. [Method] The safety concentration of Fuke Guanchang Ⅱ in Caco-2 cells were cultured by MTT assay. [Results] When the concentration above 0.1 g/L. The study showed the survival rate of Caco-2 cells were decreased with the concentration was increased. [Conclusion] It will be a valuable exploration that Caco-2 cells as a new tool to be introduced to the basic and safety research of Chinese medicine catharsis method. |
| Key words: Chinese medicine catharsis method Caco-2 cell model Fuke Guanchang Ⅱ |
