朱砂七总蒽醌对S180荷瘤小鼠凋亡基因表达的影响

Effect of total anthraquinone fromZhushaqi on expression of apoptosis gene in S180-bearing mice

关键词:  朱砂七总蒽醌  S₁₈₀瘤株  凋亡基因  c-myc  Bcl-2  p53

Key words:  anthraquinone from Zhushaqi  S₁₈₀ tumor  apoptosis gene  c-myc gene  Bcl-2 gene  p53 gene

摘要:

[目的] 观察朱砂七总蒽醌对S₁₈₀荷瘤小鼠凋亡基因c-myc、Bcl-2和突变型p53表达的影响.[方法] 建立S₁₈₀荷瘤小鼠模型,分成4组:模型对照组,阳性对照环磷酰胺组和朱砂七总蒽醌大、小剂量组,灌胃给药第11天,取各组小鼠的瘤组织,采用实时定量PCR法检测各组小鼠肿瘤组织c-myc、Bcl-2和突变型p53基因的表达.[结果] 朱砂七总蒽醌1.2g/kg和0.3g/kg剂量组c-myc、Bcl-2和突变型p53mRNA表达量较模型组均显着降低,与模型组比较有统计学意义(P<0.01或P<0.05).[结论] 朱砂七总蒽醌对S₁₈₀荷瘤小鼠的抑瘤作用可能是通过下调小鼠S₁₈₀瘤组织c-myc、Bcl-2和突变型p53mRNA的表达而实现的.

Abstract:

[Objective] To study the effect of total anthraquinone from Zhushaqi(ZSQ) on c-myc, Bcl-2 and p53 mRNA expression in S₁₈₀ tumour-bearing mice. [Methods] The S₁₈₀ mice model was established. The mice were divided into 4 groups: blank control, positive control with cyclophosphamide and ZSQ in high or low dose. Ten days after continuous treatment, all the mice were sacrificed and the tumor tissue was collected. Real-time PCR was used to determine the effect of ZSQ on the expression of c-myc, Bcl-2 and p53 mRNA. [Results] ZSQ could significantly reduce the expression of c-myc, Bcl-2 and p53 mRNA expression in S₁₈₀ tumor tissue. The average levels of c-myc, Bcl-2 and p53 expression in low-dose and high-dose groups of ZSQ had significantly difference to that in control group(P<0.01 or P<0.05). [Conclusion] Down-regulating the c-myc, Bcl-2 and p53 mRNA expression is probably one of the antitumor mechanisms of ZSQ.