| 摘要: |
| [目的] 观察透骨草提取物对人肝癌SMMC-7721细胞凋亡的影响,探讨透骨草提取物诱导SMMC-7721细胞凋亡的分子机制.[方法] 流式细胞术检测透骨草提取物诱导SMMC-7721细胞凋亡的影响;免疫组化法检测透骨草提取物对SMMC-7721细胞caspase-9、-3、-7和X连锁凋亡抑制蛋白(XIAP)表达的影响.[结果] 40.91μg/mL透骨草提取物处理的SMMC-7721细胞凋亡率为(26.84±1.23)%,明显高于对照组(P<0.05).40.91μg/mL透骨草提取物处理的SMMC-7721细胞Caspase-9、-3、-7蛋白表达明显低于与对照组(P<0.05),而XIAP蛋白表达明显高于对照组(P<0.05).[结论] 透骨草提取物能通过XIAP/Caspase信号通路抑制SMMC-7721细胞凋亡. |
| 关键词: 透骨草提取物 SMMC-7721细胞 凋亡 Caspase-9 |
| DOI：10.11656/j.issn.1672-1519.2013.10.12 |
| 分类号: |
| 基金项目:2010年大连市科学技术基金计划项目(2010E13SF144) |
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| The influences of Phryma Leptostachya L. var. asiatica Hara extract on apoptosis and apoptosis-related factors of human hepatocellular carcinoma SMMC-7721 cells |
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LI Ping1, ZENG Chang-Qian2
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1.The department of internal medicine, The people's hospital of Tumen City, Tumen 133100, China;2.Medical College of Dalian University, Dalian 116622, China
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| Abstract: |
| [Objective] To observe apoptosis of human hepatocellular carcinoma SMMC-7721 cells induced by Phryma Leptostachya L. var. asiatica Hara extract and to investigate the related molecular mechanism. [Methods] Flow cytometry was used to detect the apoptosis rate of SMMC-7721 cells induced by Phryma Leptostachya L.var. asiatica Hara extract. Immunohistochemistry technique was used to determine the expressions of caspase-9、-3、-7 and XIAP proteins of SMMC-7721 cells. [Results] The apoptotic rate of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract was(26.84±1.23)% and increased obviously than control group(P<0.05). The expressions of Caspase-9 、-3 、-7 proteins of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract decreased than control group(P<0.05). The expression of XIAP protein of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract increased than control group(P<0.05). [Conclusion] Phryma Leptostachya L. var. asiatica Hara extract could induce the apoptosis of SMMC-7721 cells by XIAP/Caspase signal pathway. |
| Key words: Phryma leptostachya L.var.asiatica Hara extract SMMC-7721 cell apoptosis Caspase-9 |
