| 摘要: |
| [目的] 观察Nogo-A在大鼠颅脑损伤后表达变化,探讨针刺干预颅脑损伤后大鼠Nogo-A表达的影响.[方法] 雄性SD大鼠随机分为针刺治疗组、模型对照组、正常对照组,用改良Feeney's打击法制成大鼠左顶叶局限性脑挫裂伤模型,分别于损伤后6、24、48h和6d组取损伤局部脑组织,采用免疫组化法观察Nogo-A的表达变化并进行统计学分析.[结果] 颅脑损伤后,从24 h开始,针刺组大鼠脑损伤组织中Nogo-A的表达开始下降.与模型组相比较,针刺组48h、6d的Nogo-A表达均显着降低,差异有统计学意义(P<0.01).[结论] 针刺治疗对大鼠颅脑损伤后Nogo-A表达具有明显的抑制作用,表明针刺对脑损伤大鼠的神经再生具有促进作用. |
| 关键词: 颅脑损伤 Nogo-A 免疫组化 针刺 |
| DOI：10.11656/j.issn.1672-1519.2013.10.13 |
| 分类号: |
| 基金项目: |
|
| Effect of acupuncture on the expression of Nogo-A in rats with craniocerebral injury |
|
TIAN Gui-hua1, Wang Xi-you1, Yao Hai-jiang2, LI Zhi-gang2
|
|
1.Department of Massage, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100029, China;2.School of acupuncture-moxibustion and tuina, Beijing University of Chinese Medicine, Beijing 100029, China
|
| Abstract: |
| [Objective] To observe variation of Nogo-A expression after craniocerebral injury(CCI)of rats,and to investigate the protective mechanism of acupuncture in CCI. [Methods] Rats were randomly divided into three groups: acupuncture group, model group and blank control group. The craniocerebral injury rat model was made by using a modified Allen's method. The craniocerebral tissues on 6, 24, 48 h and 6 d after injury were taken. The expression of Nogo-A was detected through immunohistochemistry mothods. The positive cells were analyzed by a computer image analysis system. [Results] After CCI rat model was established successfully,and the Nogo-A content of local injury tissues in acupuncture group began to decline from 24 h. Compared with the model group,statistical significance was found in the acupuncture group at 48 h, 6 d(P<0.01).[Conclusion] The acupuncture can obviously inhibit the expression of Nogo-A of the CCI rat and it is likely to play a role in the axon regeneration along the correct direction after CCI. |
| Key words: craniocerebral injury Nogo-A immunohistochemistry acupuncture |
