| 摘要: |
| [目的] 建立超高效液相色谱法同时测定13批不同产地大黄中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素等5种游离蒽醌的含量。[方法] 采用Waters BEH C18（2.1 mm×150 mm，1.8 μm），以乙腈（A）-0.1%甲酸水溶液（B）为流动相，梯度洗脱[0～3 min，19%→25%A，3～10 min，25%→100%A]，流速0.3 mL/min，柱温30 ℃，检测波长254 nm.[结果] 大黄酸、大黄酚、大黄素、大黄素甲醚、芦荟大黄素分别在1.61～80.30，4.98～249.20，3.27～163.30，9.65～482.50，1.67～83.30 μg/mL范围内呈现良好的线性关系，平均回收率分别为99.7%，100.4%，100.1%，98.9%，100.9%.相对标准偏差（RSD）分别为 1.5%，0.6%，0.8%，0.4%，1.7%.[结论] 该方法简便可行，重复性良好，可用于大黄药材的质量控制。 |
| 关键词: 大黄 含量测定 超高效液相色谱法 |
| DOI：10.11656/j.issn.1672-1519.2014.04.15 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目（30872954），天津市卫生局科技基金资助项目（2012KZ064）。 |
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| Simultaneous determination of free anthraquinones in Rhubarb fromdifferent habitats by UPLC |
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LIU Bing-wang, XU Wen-gui, Wang Jian
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Department of Molecular Imaging and Nuclear Medicine, Tianjin Medical University Cancer Institute and Hospital, National ClinicalResearch Center of Cancer, Key Laboratory of "Cancer Prevention and Therapy", Tianjin 300060, China
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| Abstract: |
| [Objective] To develop a UPLC method for simultaneous determination of five anthraquinones (Rhein,Chrysophanol,Emodin,Physcion and Aloe-emodin) in Radix et Rhizom a Rhei from different habitats. [Methods] The analysis was performed on Waters BEH C18 column (2.1 mm×150 mm,1.8 μm) with a gradient mobile phase of acetonitrile (A)-0.1% Formic acid solution (B) (0～3 min,19%→25%A,3～10 min,25%→100%A) at flow rate of 0.3 mL/min. The column was maintain at 30℃ and the detection wavelength was set at 254 nm. [Results] The linear ranges were 1.61～80.30 μg/mL for Rhein,4.98～249.20 μg/mL for Chrysophanol,3.27～163.30 μg/mL for Emodin,9.65～482.50 μg/mL for Physcion and 1.67～83.30 μg/mL for Aloe-emodin. The average recoveries of the five compounds were 99.7%,100.4%,100.1%,98.9%,100.9% and RSD were 1.5%,0.6%,0.8%,0.4%,1.7% respectively.[Conclusion] The rapid and accurate UPLC method was established. This method can be used to assess the quality of Rhubarb. |
| Key words: Rhubarb determination UPLC |
