小柴胡汤加减方对慢性胰腺炎大鼠胰腺外分泌功能的影响

Effect of modified minor Radix Bupleuri decoction on pancreatic exocrine function in rats with chronic pancreatitis

关键词:  小柴胡汤加减方  慢性胰腺炎  胰腺外分泌

Key words:  modified minor radix bupleuri decoction  chronic pancreatitis  exocrine pancreas

摘要:

[目的] 观察小柴胡汤加减方对慢性胰腺炎大鼠胰腺外分泌的影响。[方法] 体质量180～200 g健康雄性Wistar大鼠30只，随机分为3组：对照组、模型组及中药组。模型组及中药组大鼠尾静脉注射二氯二丁基酯（DBTC）溶液（7 mg/kg），对照组仅尾静脉注射相同体积的溶剂。自造模第2天起中药组给予小柴胡汤加减方灌胃（10 mL/kg），每日1次。常规饲28 d，3组大鼠行苯洛肽（BT-PABA）实验，收集6 h总尿量，计算6 h大鼠尿PABA排出率；同时留取粪便，用酶联免疫吸附试验（ELISA）法检测大鼠粪弹力蛋白酶1（FE-1）含量。之后，麻醉大鼠，下腔静脉取血，用于血清淀粉酶、脂肪酶检测；取胰腺组织，用于组织病理学观察。[结果] 组织病理学可见模型组腺泡萎缩，纤维组织增生明显，中药组病理损伤程度减轻。与对照组比较，模型组大鼠尿PABA排出率下降（P<0.05），模型组粪FE-1含量降低（P<0.05）.与模型组比较，中药组尿PABA排出率升高（P<0.05），粪FE-1含量明显增加（P<0.05）.中药组血清淀粉酶及脂肪酶活性均较模型组降低（P<0.05）.[结论] 小柴胡加减方能够改善大鼠慢性胰腺炎胰腺外分泌功能。

Abstract:

[Objective] To investigate the effects of minor Radix Bupleuri decoction on exocrine pancreatic function in rats with chronic pancreatitis. [Methods] Thirty male Wistar rats, weighing 180～200 g were randomly divided into three groups: control group,medicinal herbal group,model group. The model group and the medicinal group were injected with Dibutyltin dichloride(DBTC) solution into the caudal vein at a dose of 7 mg/kg body weight. The control group was treated with vehicle at the same volume. The medicinal herbal group lavaged with modified minor Radix Bupleuri decoction for 28 days. The n-benzoyl-tyrosyl para-aminobenzoic acid (BT-PABA) test was carried out to evaluate the exocrine pancreatic function. Feces was collected to detect the FE-1. Then rats were sacrificed and the pancreas tissue was harvested for histological examination. The activity of amylase and lipase was detected at same time. [Results] Compared with the control group, the levels of FE-1and and the excretion rate of PABA were reduced in rat with chronic pancreatitis. Compared with the model group, treatment of the modified Minor Radix Bupleuri decoction could significantly reduce the activity of amylase and lipase(P<0.05). It could increase the levels of FE-1(P<0.05) and the excretion rate of PABA. [Conclusion] The modified Minor Radix Bupleuri decoction can improve the exocrine pancreatic function in rat with chronic pancreatitis.