代料配方与传统松木栽培茯苓的质量分析比较研究

Comparative study of quality analysis between substituent cultivated Fuling and traditional pine timber cultivated Fuling

关键词:  茯苓  代料栽培  含量分析  茯苓多糖  茯苓三萜

Key words:  Fuling  substituent culture  quantitative analysis  total polysaccharides of Fuling  total triterpenoid of Filing

摘要:

[目的] 通过代料配方与传统松木栽培茯苓质量分析比对研究，考察代料栽培茯苓药材的质量。[方法] 通过紫外可见分光光度法（UV-Vis）测定茯苓的总多糖和总三萜的含量，采用高效液相色谱法（HPLC）测定茯苓有效成分茯苓酸的含量，茯苓提取物的收率通过干膏恒重法测定。[结果] 代料配方栽培茯苓各项含量测定指标普遍高于传统松木栽培茯苓，加权综合评分结果表明代料配方C 和F的综合评分并列第一，而传统松木栽培茯苓的综合评分处于末位。[结论] 代料配方与传统松木栽培茯苓各项含量指标测定的比对分析结果，为代料配方栽培茯苓的应用前景提供了参考依据，并为代料栽培茯苓的后期研究奠定了良好的基础。

Abstract:

[Objective] Through the comparative research of the quality of the substituent cultivated Fuling and traditional pine timber cultivated Fuling the content of active ingredients was analyzed and the quality of substituent cultivated Fuling was explored. [Methods] Using UV spectrophotometry (UV-Vis) the total polysaccharides and total triterpenoid were detected and the Poria acid, the effective con-tent of Fuling, was detected with RP-HPLC. The extracted ratio of Fuling was detected by dry cream constant weight measuring. [Results] Every content indicators of substituent cultivated Fuling were higher than that of pine timber cultivated Fuling. Scored with wightening showed that substituent C and F were tied for the first place, while the traditional pine timber cultivated Fuling occupied the last position. [Conclusion] The comparative studies on each indicators of substituent cultivated Fuling and pine timber cultivated Fuling have provided some reference evidences for the future application of the substituent cultivated Fuling and laid an excellent foundation for the future re-search of the substituent cultivated Fuling.