中药灌肠对5/6 肾切除大鼠肾衰竭模型微炎症状态的影响

Effect on 5/6 nephrectomy rats with micro-inflammation state treated with TCMenema

关键词:  5/6 肾切除  慢肾衰  微炎症状态  中药灌肠  辛伐他汀

Key words:  5/6 nephrectomy  chronic renal failure  micro-inflammatory state  enema  simvastatin

摘要:

[目的] 观察中药灌肠改善慢肾衰模型大鼠的微炎症状态情况，探讨其机制。[方法] 将44 只SD雄性大鼠随机分为正常对照组、模型对照组、灌肠方组和辛伐他汀组，除正除对照组外均行5/6肾切除术制作CRF 动物模型。造模后各组开始灌胃或灌肠给药6 周。分别于造模后1、3、6 周取血检测大鼠的血肌酐（SCr）、血尿素氮（BUN）、甘油三酯（TG）、C 反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）、Toll 样受4（TLR4）等变化情况。实验结束后观察肾脏和小肠组织形态学的改变。[结果] 灌肠方组大鼠的微炎症指标CRP、TNF-α浓度与辛伐他汀组没有统计学差异，说明灌肠方在改善微炎症状态中也有类似于他汀类药物的作用，其机制可能与阻断TLR4 表达有关。肾脏病理结果显示灌肠治疗对肾间质纤维化有一定的改善作用。[结论] 中药灌肠能改善CRF 大鼠生存状态，延缓病情进展，对肾间质纤维化有一定的积极作用。其作用机制可能是通过降低炎症因子表达，改善微炎症状态有关，其作用与辛伐他汀类似。

Abstract:

[Objective] To observe effect to micro-inflammation state in experimental chronic renal failure (CRF) rats treated with TCM enema and explore it's mechanism. [Methods] The 44 SD male rats were randomly divided into normal control group, model control group, enema group and simvastatin group. All rats were 5/6 nephrectomy surgery to become CRF animal models except normal control group. After model establishment, each group began to fill the stomach or enema for 6 weeks. The blood serumcreatinine (SCr), blood urea nitrogen (BUN), triglyceride (TG), C reactive protein (CRP), transforming growth factor alpha (TNF-α), and toll like receptor 4 (TLR4) were detected respectively at 1, 3, 6 weeks in each group, Animals were sacrificed at the end of the experiment to observe the morphologi-cal change of kidney and small intestine. [Results] The concentration of CRP and TNF-αwere no statistical difference between enema group and simvastatin group. It suggested that the function of TCM enema was also similar to the role of statins in improving the micro-in-flammatory state, and its mechanism may be related to blocking the expression of TLR4. Pathological results showed that enema group has a certain improving effect on renal interstitial fibrosis. [Conclusion] TCM enema can improve the CRF rat survival state, delay the progress of the disease, and have certain positive effect on renal interstitial fibrosis. The mechanism of action of TCM enema may relate to reducing inflammation factor expression, improve micro-inflammation state function. The function of TCMenema is similar to simvastatin.