丹参酮ⅡA固体脂质纳米粒的制备及质量评价

Preparation and quality evaluation of tanshinonesⅡA solid lipid nanoparticles

关键词:  丹参酮ⅡA  固体脂质纳米粒  质量评价

Key words:  TanshinonesⅡA  solid lipid nanoparticles  quality evaluation

摘要:

[目的] 以山嵛酸甘油酯和单硬脂酸甘油酯为载体材料制备丹参酮IIA固体脂质纳米粒(TA-SLN) 并评价其质量。[方法] 采用乳化固化法制备TA-SLN，以包封率为指标，通过单因素考察法对处方进行优化，激光粒径测定仪测定其粒径、电位，并用差示扫描量热仪（DSC）表征其性质。[结果] 制备的TA-SLN 平均粒径为(110±4) nm，Zeta 电位为-34.4 mV，包封率为79.08%。DSC 表明其理化性质稳定可靠。[结论] 采用乳化固化法制备的TA-SLN粒径和包封率较适宜,理化性质稳定,为开发其新制剂奠定了实验基础。

Abstract:

[Objective] To prepare tanshinonesⅡA-loaded solid lipid nanoparticles with docosanoic acid glyceride and glyceryl monos-tearate as its lipid matrix and investigate its quality. [Methods] TanshinonesⅡA-loaded solid lipid nanoparticles were prepared by emul-sion evaporation-solidification. With encapsulation efficiency as index, through the single factor the formulation was optimized, using laser particle size analyzer its particle size was measured, Using potential and use DSC its properties was characterized. [Results] The obtained solid lipid nanoparticles had an average size of (110±4) nm, zeta potential was -38.5 mV and the entrapment efficiency was 79.08%. DSC showed that its physical and chemical properties were stable and reliable. [Conclusion] The solid lipid nanoparticles prepared by the emulsion evaporation-solidification have an appropriate size, the encapsulation efficiency and stable physical and chemical properties have provided an experimental foundation for the development of the new formulation.