方剂配伍增加中药方剂活性——一项来自毒理学的实验研究

Prescription compatibility can raise activity of Chinese medicine-An experimental research fromtoxicology

关键词:  清热解毒方  合煎  分煎  LD₅₀

Key words:  Qingre Jiedu prescription  mixed-decoction  single-decoction  LD₅₀

摘要:

[目的] 比较不同制备方法对清热解毒方水煎剂毒性的影响。[方法] 清热解毒方中各味药材分别制备成不同待测样品：合煎样品、分煎样品及单味药给药样品，采用简化机率法测定各样品对小鼠灌胃给药的半数致死量（LD₅₀），并比较测定结果。[结果] 所测得合煎样品和分煎样品的LD₅₀分别为（91.26±7.92）g/kg 和（111.47±9.12）g/kg，与合煎样品比较，分煎样品的毒性显著降低（P<0.05），且动物死亡时间及死前表现不同。单味药样品给药后多未出现明显毒性反应。[结论] 与单味中药比较，复方配伍确能提高中药的生物活性。

Abstract:

[Objective] To compare the effect of toxicity of Qingre Jiedu(clearing heat and detoxification) prescription in different prepa-ration method. [Methods] The drugs contained in Qingre Jiedu prescription were prepared to different samples: single-decoction, mixed-decoction, and single-drug-decoction. The LD₅₀ of each sample after intragastric administration was determined in mice by simplified probit method, and the results were compared. [Results] The LD₅₀ of mixed-decoction and single-decoction samples were (91.26±7.92) g/kg and(111.47±9.12)g/kg respectively. Compared with the mixed-decoction samples, the toxicity of single-decoction samples was de-creased significantly (P<0.05). Different death time and different behaviors before death were fund in animals. Single-drug-decoction sam-ples showed no toxic reaction in most animals after administration. [Conclusion] Compared with single-decoction sample and single-drug-decoction sample, compatible prescription has a certain effect of raising the bioactivity of Chinese medicine.