| 摘要: |
| [目的] 研究黄芪甲苷对肝缺血/再灌注损伤的保护作用及其机制。[方法] 将雄性SD大鼠36只，随机分为3组：假手术组（Sham组）、肝缺血/再灌注组（I/R组）、黄芪甲苷预处理组（AsIV组）.分光光度法测定大鼠血清谷丙转氨酶（ALT）、谷草转氨酶 （AST）活性；苏木精-伊红（HE）染色观察肝组织形态学变化，蛋白印迹法（Western blot）检测心肌组织内Bax，Bcl-2蛋白表达。[结果] 与假手术组比较，肝I/R组ALT、AST活性显着增高，Bax蛋白表达增加，Bcl-2蛋白表达降低，黄芪甲苷预处理可以降低肝缺血/再灌注损伤引起的ALT、AST活性增高，Bax蛋白表达增加，增加Bcl-2蛋白表达。[结论] 黄芪甲苷预处理对肝缺血/再灌注损伤有一定的保护作用，其机制可能与黄芪甲苷抑制肝细胞凋亡有关。 |
| 关键词: 黄芪甲苷 肝缺血/再灌注损伤 细胞凋亡 |
| DOI：10.11656/j.issn.1672-1519.2014.10.12 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目（81173443）；辽宁省教育厅资助科研资助项目（LJQ2011087）. |
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| Protective effect of Astragaloside IV against liver ischemia and reperfusion injury in rats |
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LI Tie-cheng, MA Bao-feng, LI De-sheng, AI Li-li, AI Hao
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The Third Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, China
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| Abstract: |
| [Objective] To investigate the protective effect of Astragaloside IV (As IV) against liver ischemia and reperfusion injury and the underlying mechanism. [Methods] The 36 SD rats were randomly divided into 3 groups: sham operation group (Sham group), liver ischemia-reperfusion (I/R group) group and As IV group. The content of ALT and AST were measured by spectrophotometric method. Liver pathological changes were observed by HE staining. Bax and Bcl-2 protein expression was determined by Western blot. [Results] Compared with sham group, the content of ALT and AST was significantly increased, while the Bcl-2 protein expression decreased and the Bax expression increased. Pretreatment of As IV before liver I/R could decrease the content of ALT and AST, Bax expression, and increase the Bcl-2 protein expression (P<0.05). [Conclusion] As IV pretreatment has a protective effect against liver ischemia reperfusion injury, which is partially finished via inhibiting liver cell apoptosis. |
| Key words: Astragaloside IV liver ischemia reperfusion injury cell apoptosis |
