| 摘要: |
| [目的] 建立同时测定人参流动浸膏中8种人参皂苷类成分含量的超高效液相色谱法（UPLC）方法。[方法] 采用Waters Acqutity UPLC色谱系统，Acqutity UPLC®BEH C18（1.7 μm，2.1 mm×50 mm）色谱柱，流动相为乙腈-水，梯度洗脱，流速0.6 mL/min，柱温40 ℃，检测波长203 nm.[结果] 人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rb3和Rd分别在0.009 7~0.291 0 μg，0.008 5~0.255 0 μg，0.0075~0.300 0 μg，0.010 4~0.416 0 μg，0.010 7~0.428 0 μg，0.007 4~0.296 0 μg，0.004 2~0.168 0 μg和0.009 1~0.364 0 μg 范围内成良好的线性关系，平均回收率分别为101.4%、101.1%、100.1%、99.8%、99.3%、98.8%、98.8%和100.5%.[结论] 方法快速、准确、重复性好，可为人参流动浸膏的质量控制提供快速准确的检测方法。 |
| 关键词: 人参流动浸膏 人参皂苷 UPLC 含量测定 |
| DOI：10.11656/j.issn.1672-1519.2014.10.13 |
| 分类号: |
| 基金项目:科技创新体系及条件平台建设计划项目（12TXGCCX03800）. |
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| Determination of eight ginsenosides in Ginseng extract by UPLC |
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LI Wei1,2, JI Li-na1, SONG Xin-bo1,2
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin Zhong Yi Pharmaceutical Co., Ltd., Tianjin 300193, China
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| Abstract: |
| [Objective] To develop a ultra-high performance liquid chromatography method for simultaneously determination of eight ginsenosides in Ginseng extract. [Methods] The analysis was performed on a Waters Acqutity UPLC system eluted with mobile phases of acetonitrile and water in gradient mode. The column was Acqutity UPLC®BEH C18 (1.7 μm, 2.1 mm×50 mm). The flow rate was 0.6 mL/min. The column temperature was set at 40 ℃ and the detection wavelength was set at 203 nm. [Results] Good linearity was obtained in the range of 0.009 7~0.291 0 μg, 0.008 5~0.255 0 μg, 0.007 5~0.300 0 μg, 0.010 4~0.416 0 μg, 0.010 7~0.428 0 μg, 0.007 4~0.296 0 μg, 0.004 2~0.168 0 μg and 0.009 1~0.364 0 μg for ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3 and Rd respectively. The method recoveries of eight compounds were 101.4%, 101.1%, 100.1%, 99.8%, 99.3%, 98.8%, 98.8% and 100.5% respectively. [Conclusion] The established method can rapidly receive an accurate and reproducible result used for controlling the quality of Ginseng extract. |
| Key words: Ginseng extract ginsenoside UPLC assay |
