扶肾降浊方对肾小管间质损害大鼠抗纤维化因子HGF、BMP-7的影响

Effect of Fushen Jiangzhuo formula on anti-fibrosis factors of HGF and BMP-7 in rats with renal tubulointerstitial lesion

关键词:  扶肾降浊方  肾小管间质损害  肝细胞生长因子  骨形态发生蛋白-7

Key words:  Fushen Jiangzhuo formula  renal tubulointerstitial lesion  hepatocyte growth factor  bone morphogenetic protein-7

摘要:

[目的] 探讨扶肾降浊方对系膜增生性肾小球肾炎(MsPGN)大鼠肾小管间质损害的疗效机制.[方法] 在MsPGN动物模型基础上,延长造模时间至20周,使其自然发展为肾小管间质损害模型.实验设中药组、西药组、模型组及空白组.中药组给予扶肾降浊方,西药组给予贝那普利,模型组和空白组给予生理盐水,连续20周,分别于实验第12、16和20周末取材,采用实时逆转录-聚合酶链反应(RT-PCR)和免疫组织化学法检测大鼠肾组织抗纤维化因子肝细胞生长因子(HGF)、骨形态发生蛋白-7(BMP-7) mRNA及蛋白表达水平.[结果] 模型大鼠肾组织HGF、BMP-7 mRNA和蛋白表达下调,扶肾降浊方随着给药周期的延长可部分逆转间质损害造成的上述mRNA和蛋白表达异常.[结论] 扶肾降浊方对MsPGN大鼠肾小管间质损害保护作用机制可能与调节抗纤维化因子HGF、BMP-7 mRNA和蛋白表达有关.

Abstract:

[Objective] To study the protective effects and mechanism of Fushen Jiangzhuo formula (FSJZ) on renal tubulointerstitial lesion in rats. [Methods] The Mesangial proliferative glomerulonephritis (MsPGN) in rats was produced and through extending the time of the modeling to 20th weeks the model was naturally developed to tubulointerstitial lesion. The rats were divided into 4 groups, including Chinese medicine group, Western medicine positive control group, model group and blank group. FSJZ formula was given to the rats of Chinese medicine group and benazepril was given to Western medicine group, while the same volume of saline was given to the animals in model group and blank group for 20 weeks. We isolated the kidney tissue of the rats at the end of 12, 16, 20 week and observed the effect of FSJZ on anti fibrosis factors of HGF and BMP-7 in renal tissue by real-time reverse transcription-polymerase chain reaction and immunohistochemistry method. [Results] The anti-fibrosis factors of HGF and BMP-7 changed in renal tubulointerstitial lesion in rats. Renal tubulointerstitial lesion could significantly down-regulate the expressions of HGF and BMP-7, while FSJZ could significantly up-regulate the expressions of HGF and BMP-7 and antagonize these effects caused by renal tubulointerstitial lesion. [Conclusion] FSJZ has distinct effects of protecting pathological renal interstitial fibroblasts. The mechanism may be related with the regulating effect of FSJZ on mRNA and protein expression of HGF and BMP-7.