| 摘要: |
| [目的] 建立甘草多糖分子量及分子量分布的测定方法,为甘草多糖质量控制提供方法. [方法] 利用高效凝胶色谱法测定甘草多糖的分子量及分子量分布;色谱柱为TOSOH TSK gel G4000 PWXL凝胶色谱柱,检测器为示差折光检测器,流动相为0.7%硫酸钠水溶液,柱温35 ℃,流速为0.8 mL/min,进样量20 μL.[结果] 6批样品的重均分子量在8.0×104~1.0×105之间,线性关系良好,精密度、重复性均良好.[结论] 本实验方法操作简便、快速、准确,为甘草多糖规模化生产的质量控制提供了参考. |
| 关键词: 高效凝胶色谱法 甘草多糖 分子量 质量控制 |
| DOI：10.11656/j.issn.1672-1519.2015.01.13 |
| 分类号: |
| 基金项目:国家科技支撑计划基金资助项目(2011 BAI07B02-5). |
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| Determination of molecular weight and weight distribution in Licorice Polysaccharide by HPGPC |
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ZHAO Ying1, SONG Xin-bo1, ZHANG Li-juan1, YU Teng-fei2
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Inner Mongolia Medical College, Hohhot 010000, China
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| Abstract: |
| [Objective] To establish a method for quality control of Licorice polysaccharide. [Methods] Using high performance gel permeation chromatography (HPGPC) for determining the Molecular Weight (MW) and Molecular Weight Distribution of Licorice Polysaccharide; TOSOH TSK gel G4000 PWXL was used as column. Mobile phase was 0.7% Na2SO4 aqueous solution at 0.8 mL/min, column temperature at 40 ℃, injection volume as 20 μL. Detector was differential refraction detector. [Results] The MW of Licorice Polysaccharides was 8.0×104~1.0×105 with good linearity, precision and reproducibility. [Conclusion] This determination method is simple, rapid and accurate. It provides a reference for the quality control of licorice polysaccharide large-scale production. |
| Key words: high performance gel permeation chromatography Licorice Polysaccharide molecular weight quality control |
