减味银翘散复方挥发油的抗菌性研究

Study on antibacterial activities of compound volatile oil extracted from Jianwei Yinqia powder

关键词:  减味银翘散  复方挥发油  抗菌性  β-环糊精包合

Key words:  Jianwei Yinqiao powder  compound volatile oil  antibacterial activity  β-cyclodextrin inclusion

摘要:

[目的] 采用"试管比浊法"研究减味银翘散复方挥发油β-环糊精包合前后对大肠杆菌和金黄色葡萄球菌的抗菌性.[方法] 固定大肠杆菌和金黄色葡萄球菌的浓度,分别加入不同浓度的复方挥发油溶液,测定挥发油不同浓度下的抑菌率,得到复方挥发油的最小抑菌浓度(MIC)和最小杀菌浓度(MBC).[结果] 该复方挥发油乙醇溶液4 ℃避光在20 h内最大吸收波长257 nm处吸光度与初始吸光度无统计学差异(P<0.05),表明挥发油在20 h内比较稳定.采用β-环糊精包合来增强复方挥发油的稳定性.包合前复方挥发油对大肠杆菌和金黄色葡萄球菌均有较好的抗菌能力,对大肠杆菌的MIC为1.25、5 μL/mL,对金黄色葡萄球菌的MIC为0.63、10 μL/mL.β-环糊精复方挥发油包合物在液体培养基中37 ℃溶出后,对大肠杆菌和金葡萄球菌抑菌率分别为39.1%和23.4%.[结论] 减味银翘散复方挥发油对大肠杆菌和金黄色葡萄球菌具有较好的体外抑菌作用,利用β-环糊精包合能增强复方挥发油的稳定性,包合后挥发油对上述两种革兰氏菌仍有较强的抗菌性,为银翘散复方挥发油的进一步开发和利用提供参考.

Abstract:

[Objective] To study the antibacterial activities for Escherichia coli and Staphylococcus aureus of the compound volatile oil extracted from the compound recipe Jianwei Yinqiao powder and its β-cyclodextrin inclusion compound with turbid dtermination in test tube. [Methods] The concentration of Escherichia coli or Staphylococcous aureus was fixed and different concentrations of the volatile oil were added into the above bacteria solution, then the inhibition rates of the compound volatile oil with different concentrations were determined, and the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the compound volatile oil were calculated. [Results] The absorbance of the volatile oil ethanol solution preserved at 4℃ and protected from light had no significant differences(P<0.05)from the initial absorbance at the maximum absorption wavelength 257 nm within 20 h, which showed that the compound volatile oil was relatively stable within 20 h. The compound volatile oil was included by β-cyclodextrin to increase the stability of the compound volatile oil solution. The compound volatile oil had good antibacterial activities against Escherichia coli and Staphylococcus aureus before or after included, and the MIC and the MBC of volatile oil against Escherichia coli were about 1.25 μL/mL and 5 μL/mL. The MIC and the MBC against Staphylococcus aureus were about 0.63 μL/mL and 10 μL/mL. After the β-cyclodextrin inclusion of the compound volatile oil was dissolved in the liquid medium at 37 ℃, the inhibition rate for Escherichia coli and Staphylococcus aureus were 39.1% and 23.4% respectively. [Conclusion] The compound volatile oil extracted from Jianwei Yinqiao powder has good inhibitory activities against Escherichia coli and Staphylococcus aureus in vitro. The compound volatile oil was included by β-cyclodextrin to increase the stability of the volatile oil collusion and the β-cyclodextrininclusion of the compound volatile oil still has a strong antibacterial activity against the above gram-bacteria. The study should provide a reference for the further development and use of the compound essential oil from Yinqiaosan decoction.