穴位局部TLR4在针刺后穴位局部炎症反应中作用的初步研究

Preliminary research on the role of TLR4 at acupoints in the inflammatory reaction produced by acupuncture

关键词:  针刺  穴位  TOLL样受体4  炎症反应

Key words:  acupuncture  acupuncture point  TLR4  inflammatory reaction

摘要:

[目的] 探讨穴位局部TOLL 样受体4(TLR4)在针刺后穴位局部炎症反应中的作用。[方法] 将80 只健康大鼠随机分为针刺组和空白对照组,每组各40 只。每组的40只大鼠再随机分8 个时点(即刻、15 min、30 min、1 h、2 h、4 h、8 h、24 h),每个时点5只。采用ELISA 法检测各时间点穴区皮肤肌肉组织中TLR4、高迁移率族蛋白(HMGB1)、白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的浓度。[结果] 空白对照组,各指标浓度在各时间点均无明显变化;针刺组针刺后TLR4、HMGB1、IL-6、TNF-α浓度升高,而IL-1β浓度无明显变化。对每只大鼠穴位局部TLR4 与其他指标浓度的相关性进行分析发现,针刺后HMGB1、TNF-α与TLR4 的相关性增强,IL-6 与TLR4 的相关性减弱,IL-1β与TLR4 的相关性针刺前后则无明显变化。[结论] 穴位局部TLR4 介导的通路可能是针刺后穴位局部炎症反应产生的通路之一。

Abstract:

[Objective] To investigate the role of TLR4 at acupoints in the inflammatory reaction produced by acupuncture.[Methods] The 80 health rats were divided into acupuncture group and blank control group, each group 40 rats. Each group was randomly divided into 8 time points(immediate, 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 24 h), 5 rats per point. Testing the concentrations of TLR4, HMGB1, IL-1α, IL-6, TNF-βin point area including skin and muscle tissue with ELISA. [Results] There were no significant changes in the concentration of each index at each time point in control group. Acupuncture could increase the concentrations of TLR4, HMGB1, IL-6, TNF-β, whereas IL-1αconcentrations did not change. The concentrations of HMGB1, TNF-βhad certain relativity with the concentration of TLR4 respectively after acupuncture. However, the concentration of IL-6 had weaken relativity with TLR4and the concentration of IL-1αhad uncertain relativity with TLR4. [Conclusion] TLR4 located at acupoints may play an important part in the inflammatory reaction produced by acupuncture.