探讨益肝降脂方对大鼠酒精性脂肪肝病理影响与疗效的相关性

Investigation on the correlation of the effect of Yigan Jiangzhi Fang on pathology in rats with alcoholic fatty liver

关键词:  益肝降脂方  酒精性脂肪肝  肝组织病理

Key words:  Yigan Jiangzhi Fang  alcoholic fatty liver  hepatic tissue pathology

摘要:

[目的] 通过观察肝脏组织形态学的改变以探讨求证益肝降脂方(YGJZF)对酒精性脂肪肝大鼠肝组织病理的影响及分析。[方法] 实验共分为3 期,共245 只雄性SD大鼠分为3 个大组:空白组15 只、模型组50 只、中药组180 只。统一采用高脂饲料+乙醇灌胃法建立大鼠酒精性脂肪肝模型,并分别予以相应中药干预,获取各组大鼠肝脏同一位置组织制作苏木精-×红(HE)染色病理切片,观察3 组大鼠肝脏组织形态学的变化。[结果] 中药各组大鼠肝组织损伤程度均较模型组轻,且两者存在显著差异性(p<0.05)。[结论] YGJZF 能够减轻酒精性脂肪肝大鼠肝组织的病理损伤,对酒精性脂肪肝有肯定的保护作用。

Abstract:

[Objective] To observe the morphological changes of liver tissue in order to discuss and verify the pathology effects on liver tissue of Yigan Jiangzhi Fang (YGJZF) on alcoholic fatty liver in rats. [Methods] The experiments were divided into 3 stages. A total of 245 male SD rats were randomly divided into 3 groups: blank group 15, model group 50, YGJZF group 180. Rats were given fat diet and alcohol feeding to establish alcoholic fatty liver model, and were separately corresponding intervention, rats in each group were stained with HE to make pathological slices at the same position of liver tissue, observed the changes of morphological liver tissue of three groups of rats. [Results] The degree of rats liver tissue injury in YGJZF group was lighter than in the model group, and the difference was statistically significant. [Conclusion] YGJZF can reduce the pathological injury of liver tissue of alcoholic fatty liver in rats, has a positive protection effect on alcoholic fatty liver.