| 摘要: |
| [目的] 观察心复康协同骨髓间充质干细胞(BMSC)对心脏干细胞(CSC)GATA4和Cx43 mRNA表达的调节。[方法] 采用全骨髓贴壁法获得BMSC,并对其进行流式细胞鉴定。制备心复康含药血清,高效液相色谱分析其成分,并作用于细胞共培养体系,采用实时定量PCR法检测各组CSC中GATA4和Cx43 mRNA的表达。[结果] 相较于其他两组,心复康组CSC中的GATA4和Cx43 mRNA表达明显增加,差异有统计学意义(P<0.05).[结论] 心复康协同BMSC能够上调CSC中GATA4和Cx43 mRNA的表达。 |
| 关键词: 心复康 骨髓间充质干细胞 心脏干细胞 GATA结合蛋白4 缝隙连接蛋白43 |
| DOI：10.11656/j.issn.1672-1519.2015.05.10 |
| 分类号: |
| 基金项目:国家自然科学基金项目(81173413). |
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| The synergy between Xinfukang and Bone marrow mesenchymal stem cells increases the expression of GATA4 and Cx43 in cardiac stem cells |
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WANG Yi-jing1, SUO Yan-rong1, ZENG Wen-yun1, KAN Bo-hong2, JIANG Xi-juan1, FAN Ying-chang1
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.The First Affiliated Hospital of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the synergy effects between Xinfukang and Bone marrow mesenchymal stem cells(BMSC) in the expression of GATA4 and Cx43 in Cardiac stem cells(CSC). [Methods] BMSC were obtaining by the whole bone marrow adherent method, and the purity was evaluated by flow cytometry. Xinfukang convalescent serum were analyzed through high performance liquid chromatography(HPLC), and then used for cell co-culture system. Real-time quantitative PCR were used to detect the expression of GATA4 and Cx43 in groups of CSC. [Results] GATA4 and Cx43 in the Xinfukang group were increased significantly(P<0.05). [Conclusion] Xinfukang collaborative with BMSC can increase the expression of GATA4 and Cx43in CSC. |
| Key words: Xinfukang bone marrow mesenchymal stem cell cardiac stem cell GATA4 Cx43 |
