甘草酸对葛根素增溶作用的实验研究

Research of the solubilization of glycyrrhizic acid to puerarin

关键词:  甘草酸  葛根素  增溶  高效液相色谱法  表面张力  pH值

Key words:  glycyrrhizic acid  puerarin  solubilization  HPLC  surface tension  pH value

摘要:

[目的] 通过测定葛根、甘草单煎液和其不同配伍比例的合煎液中葛根素和甘草酸的含量及表面张力,计算甘草酸的临界胶束浓度,探讨甘草酸对葛根素增溶作用的机制。[方法] 采用HPLC测定葛根素和甘草酸的含量,采用Wilhelmy吊片法测定溶液的表面张力。[结果] 当葛根和甘草的比例为5∶3时,葛根素的溶出度最大,此时甘草酸的临界胶束浓度是0.18 g/L,临界胶束浓度与加入药物的结构和溶液的pH值有关。[结论] 甘草酸具有表面活性,达到临界胶束浓度可以增加葛根素的溶解度。

Abstract:

[Objective] To calculate the critical micelle concentration of glycyrrhizic acid and explore the solubilizing mechanism of glycyrrhizic acid to puerarin, by measuring the amount of puerarin and glycyrrhizic acid and the surface tension of solution in single decoction of pueraria and glycyrrhiza and their mixed decoction of different proportions. [Methods] The HPLC procedure was performed for measuring the amount of puerarin and glycyrrhizic acid, and the surface tension of solution was measured by Wilhelmy plate method. [Results] When the ratio of pueraria and glycyrrhiza was 5:3, the dissolution of puerarin reached maximum, and the critical micelle concentration of glycyrrhizic acid was 0.18 g/L, which was affected by the structure of drug and the pH value of solution.[Conclusion] Glycyrrhizic acid has surface activity, and it can increase the solubility of puerarin when its concentration reaches the critical micelle concentration.