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| 丹参酮Ⅰ脂质体的制备及表征 |
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郭丽丽1,2, 范丽丽1,2, 王爱潮1,2, 庞晓晨1,2, 罗配1,2, 杨红云1,3, 王少峡1,3, 刘志东1,2
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1.天津市现代中药重点实验室, 天津中医药大学, 天津300193;2.天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津300193;3.天津中医药大学中医药研究院, 天津市现代中药重点实验室——省部共建国家重点实验室, 天津市中药药理学重点实验室, 天津300193
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| 摘要: |
| [目的] 制备丹参酮Ⅰ脂质体,并对其包封率、粒径、电位等理化性质进行考察。[方法] 采用薄膜分散法丹参酮Ⅰ脂质体,用琼脂糖凝胶柱色谱法和紫外分光光度法测定包封率,用差示扫描量热法检测丹参酮Ⅰ脂质体各组成物质的相变过程。[结果] 丹参酮Ⅰ在1.004~6.024 μg/mL范围内线性关系良好(r=0.999 9),琼脂糖凝胶柱色谱法能有效分离脂质体和游离药物,加样回收率为(98.23±0.02)%,平均包封率为(92.56±0.39)%,粒径为(90.64±1.21) nm,电位为(-35.37±0.84) mV.[结论] 薄膜分散法可用于制备丹参酮Ⅰ脂质体,制备的丹参酮Ⅰ脂质体的包封率高,粒径均一,电位稳定,药物含量和包封率测定方法准确可靠,专属性强。 |
| 关键词: 丹参酮Ⅰ 脂质体 包封率 |
| DOI：10.11656/j.issn.1672-1519.2015.05.14 |
| 分类号: |
| 基金项目:教育部新世纪优秀人才支持计划(NCET-12-1068). |
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| Preparation and characterization of TanshinoneⅠ-loaded Liposomes |
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GUO Li-li1,2, FAN Li-li1,2, WANG Ai-chao1,2, PANG Xiao-chen1,2, LUO Pei1,2, YANG Hong-yun1,3, WANG Shao-xia1,3, LIU Zhi-dong1,2
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1.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.Insitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To prepare TanshinoneⅠ-loaded Liposomes (TSⅠ-LP) and investigate its entrapment efficiency (EE), particle size, Zeta potential and other physicochemical properties. [Methods] TSⅠ-LP were prepared by film dispersion method, and using Sepharose gel and UV spectrophotometry to determine the entrapment efficiency of TSⅠ-LP. Differential scanning calorimetry (DSC) thermograms of the liposomal samples were recorded respectively. [Results] A good linear relationship was showed of TSⅠin the range of 1.004~6.024 μg/mL (r=0.999 9). Sepharose gel was well in separating the liposomes from free TSⅠ. The average recovery rate was (98.23±0.02)%. The EE of TSⅠ-LP was (92.56±0.39)%. The particle size was (90.64±1.21) nm and the Zeta potential was (-35.37±0.84) mV. [Conclusion] The film dispersion method can be used to prepare TSⅠ-LP with a high EE, a narrow size distribution and a stationary potential. The method which determine the content of TSⅠ-LP is reliable and sensitive. |
| Key words: TanshinoneⅠ Liposomes entrapment efficiency |
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